文化大學機構典藏 CCUR:Item 987654321/41921
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    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/41921


    题名: Survey of genetic structure of geese using novel microsatellite markers
    作者: Lai, FY (Lai, Fang-Yu)
    Tu, PA (Tu, Po-An)
    Ding, ST (Ding, Shih-Torng)
    Lin, MJ (Lin, Min-Jung)
    Chang, SC (Chang, Shen-Chang)
    Lin, EC (Lin, En-Chung)
    Lo, LL (Lo, Ling-Ling)
    Wang, PH (Wang, Pei-Hwa)
    贡献者: 動物科學系
    关键词: POPULATION-STRUCTURE
    PHYLOGENETIC TREES
    DATA SETS
    DNA LOCI
    DIVERSITY
    GOOSE
    SOFTWARE
    PROGRAM
    ORIGIN
    日期: 2018-02
    上传时间: 2019-01-21 15:37:36 (UTC+8)
    摘要: Objective: The aim of this study was to create a set of microsatellite markers with high polymorphism for the genetic monitoring and genetic structure analysis of local goose populations.

    Methods: Novel microsatellite markers were isolated from the genomic DNA of white Roman geese using short tandem repeated probes. The DNA segments, including short tandem repeats, were tested for their variability among four populations of geese from the Changhua Animal Propagation Station (CAPS). The selected microsatellite markers could then be used to monitor genetic variability and study the genetic structures of geese from local geese farms.

    Results: 14 novel microsatellite loci were isolated. In addition to seven known loci, two multiplex sets were constructed for the detection of genetic variations in geese populations. The average of allele number, the effective number of alleles, the observed heterozygosity, the expected heterozygosity, and the polymorphism information content were 11.09, 5.145, 0.499, 0.745, and 0.705, respectively. The results of analysis of molecular variance and principal component analysis indicated a contracting white Roman cluster and a spreading Chinese cluster. In white Roman populations, the CAPS populations were depleted to roughly two clusters when K was set equal to 6 in the Bayesian cluster analysis. The founders of private farm populations had a similar genetic structure. Among the Chinese geese populations, the CAPS populations and private populations represented different clads of the phylogenetic tree and individuals from the private populations had uneven genetic characteristics according to various analyses.

    Conclusion: Based on this study's analyses, we suggest that the CAPS should institute a proper breeding strategy for white Roman geese to avoid further clustering. In addition, for preservation and stable quality, the Chinese geese in the CAPS and the aforementioned proper breeding scheme should be introduced to geese breeders.
    显示于类别:[動物科學系 ] 期刊論文

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