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https://irlib.pccu.edu.tw/handle/987654321/41894
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| Title: | Characteristics of fucose-containing polysaccharides from submerged fermentation of Agaricus blazei Murill |
| Authors: | Wang, HT (Wang, Hsueh-Ting)
Yang, LC (Yang, Li-Chan)
Yu, HC (Yu, Hui-Ching)
Chen, ML (Chen, Miaw-Ling)
Wang, HJ (Wang, Huei-Ju)
Lu, TJ (Lu, Ting-Jang) |
| Contributors: | 生活應用科學系暨生活應用科學研究所 |
| Keywords: | MEDIATED ANTITUMOR POLYSACCHARIDES
STRUCTURAL-CHARACTERIZATION
GANODERMA-LUCIDUM
NMR-SPECTROSCOPY
FRUITING BODY
CELLS
HIMEMATSUTAKE
BIOSYNTHESIS
ELUCIDATION
ACTIVATION |
| Date: | 2018-04 |
| Issue Date: | 2019-01-17 16:20:40 (UTC+8) |
| Abstract: | Fucose is one of important residues of recognition pattern for many immune cells. In this study, we characterized bioactive fucose-containing acidic polysaccharides from submerged fermentation of Agaricus blazei Murill. We obtained the polysaccharides through a cell-based activity-guided strategy, and used carbohydrate recognition monoclonal antibodies based Enzyme-Linked Immuno Sorbent Assay (ELISA) along with methylation and NMR analyses to investigate the structural characteristics of the polysaccharides. The polysaccharides had Mw of 3.5 x 10(5) Da. The major sugars were L-fucose, L-arabinose, Dgalactose, D-xylose, and D-galacturonic acid in the molar ratio of 6.4, 15.5, 28.5, 14.7, and 25.0% with a small amount of D-glucose, D-mannose, L-rhamnose, and D-glucuronic acid. Results indicated that the bioactive polysaccharides consisted of a (1,4)-Galp and (1,4)GalAp back bone; (1,2)-Xyl and (1,2)-Rha might also comprise backbone or constitute side chain; linkage (1,5)-Ara and terminal fucosyl residues were also involved in the polysaccharides. Regarding bioactivity, removal of the terminal L-fucosyl residues reduced the TNF-alpha cytokine stimulating activity of the polysaccharides in a RAW 264.7 macrophage cell-line test, whereas NF-kappa B and TLR4 affected the polysaccharide-induced TNF-alpha production. Copyright (c) 2017, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license. |
| Appears in Collections: | [Department of Applied Science of Living & Graduate Institute of Applied Science of Living ] journal articles
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