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https://irlib.pccu.edu.tw/handle/987654321/41880
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| Title: | Human Kinase/Phosphatase-Wide RNAi Screening Identified Checkpoint Kinase 2 as a Cellular Factor Facilitating Japanese Encephalitis Virus Infection |
| Authors: | Chan, YL (Chan, Yi-Lin)
Liao, CL (Liao, Ching-Len)
Lin, YL (Lin, Yi-Ling) |
| Contributors: | 生科系 |
| Keywords: | DNA-DAMAGE RESPONSE
CYCLE ARREST
REPAIR PROTEINS
G(0)/G(1) PHASE
HOST FACTORS
REPLICATION
ACTIVATION
PATHWAY
CELLS
ATM |
| Date: | 2018-05-17 |
| Issue Date: | 2019-01-17 15:08:24 (UTC+8) |
| Abstract: | Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, causes acute encephalitis in humans with high mortality. Not much is known about the interactions between viral and cellular factors that regulate JEV infection. By using a kinase/phosphatase-wide RNAi screening approach, we identified a cell cycle-regulating molecule, checkpoint kinase 2 (CHK2), that plays a role in regulating JEV replication. JEV infection induced G1 arrest and activated CHK2. Inactivation of CHK2 and its upstream ataxia-telangiectasia mutated kinase in JEV-infected cells by using inhibitors reduced virus replication. Likewise, JEV replication was significantly decreased by knockdown of CHK2 expression with shRNA-producing lentiviral transduction. We identified CHK2 as a cellular factor participating in JEV replication, for a new strategy in addressing JEV infection. |
| Relation: | Frontiers in Cellular and Infection Microbiology 開放取用卷 8, 期 MAY 17 May 2018 論文號碼 142 |
| Appears in Collections: | [Department of Biology ] journal articles
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