| 摘要: | 多重測定感測平台，是能夠同時檢測多個生物標記分子，由於疾病的高度異質性和治療反應，對於臨床需要變得越來越重要。金屬奈米粒子由於具有獨特的侷域表面電漿共振(Localized Surface Plasmon Resonance, LSPR)現象，顯示與粒子的形狀和材料以及周圍介質相關的LSPR消光光譜特性，因此相當有潛力作為多重測定的方法。目前已經證實金屬奈米結構的LSPR相位也受到奈米結構的形狀和材料影響，由於相位受到入射電場強度不穩定性的影響較小，所以相位偵測法可以展現較低的偵測極限，在平面式的表面電漿共振傳感器已經得到證實。但是奈米結構的製作步驟繁瑣而且儀器成本昂貴，更重要的是無法同時存在多種形狀和材料的奈米結構在同一區域，所以無法實現多重測定的目的。本三年計畫為研究膠體金屬奈米粒子的LSPR之相位與頻譜的關係，針對粒子的大小、形狀、材料作系統性探討，並以此為依據，建構新型相位感測式侷域表面電漿共振傳感器，一個基於空間相位調制技術的動態偏振式干涉儀，可以一次取得四種相位移，不需要特別抗震動的架構，並利用最簡單的奈米膠體溶液之LSPR作為偵測的機制，搭配計畫中建構的分析模型，計算出各種待測物的濃度。此構想打造了一個方便性高、操作簡單而且高靈敏度的量測平台供生物多重測定之研究。
Multiplex detection, which are able to detect multiple targets simultaneously, has become increasingly important for clinical needs, in the last decade, because of the high heterogeneities of disease and therapeutic response. The unique and outstanding optical properties of metallic nanoparticles, so called localized surface plasmon resonance (LSPR), exhibit a wavelength-dependent extinction, which relies on their sizes, shapes, dielectric constants, and surrounding environments, such that they have potential to be implemented for multiple target analysis. To date, it is known that the phase of the plasmonic nanostructures is also affected by their structure and dielectric constants. Due to the less impact on phase by the intensity instability of the incident field, phase interrogation would perform better sensing ability than conventional extinction spectra, as shown for conventional SPR sensing. However, the fabrication processing of the nanostructures is complicated and the high fabrication cost is an issue as well. Most importantly, the nanostructures with various shapes and materials cannot be fabricated on the same chip area such that multiple target detection is disable to be performed. This proposal aims to study systematically the correlation between phase and spectrum of colloidal-based LSPR via particle size, shape, material. In accordance with the results, a novel phase-sensitive LSPR biosensor will be designed and constructed based on a vibration-insensitive dynamic polarization interferometry. In combination with colloidal-based LSPR and an analytical mode for spectral crosstalk correction to achieve multiplex LSPR assays. This innovative approach offers an convenient, easy-to-use and highly sensitive measurement platform for biomonitoring-related research. |
