| 摘要: | 本研究中利用超臨界二氧化碳從南瓜子中進行精油的萃取，探討壓力與溫度對精油產率的影響，在本實驗中運用中央合成設計(CCD)，每組實驗的條件因子都具有五種水準，在實驗過程中，實驗設計的壓力條件為2500 psi、2700psi、3000 psi、3200psi、3500 psi，溫度條件為40℃、42℃、45 ℃、48℃、50 ℃，因本實驗為2因子之中央合成設計，故有4個角點，4個軸點以及重複5個中央點實驗。在實驗結束後，對實驗結果進行SPSS軟體進行二階迴歸分析，探討精油的產率在壓力項、溫度項、壓力平方項、溫度平方項、壓力和溫度交互作用項具有顯著性，並且使用Matlab作圖軟體作圖獲得迴歸線圖，最後再對二階模型進行分析得到本實驗翻為中之最佳操作條件及最佳的產率，之後對比使用索氏萃取法進行南瓜子精油的租脂肪率萃取，與超臨界萃取法所得的南瓜子精油產率是否依一致，並使用氣相層析質譜儀進行分析兩者所得產物中脂肪酸是否也一致。
In this study, supercritical carbon dioxide was used to extract essential oils from pumpkin seeds, and the effects of pressure and temperature on the yield of essential oils were investigated. In this experiment, central synthesis design (CCD) was used. The condition factors of each experiment were of five levels. During the experiment, the experimental design pressure conditions were 2500 psi, 2700 psi, 3000 psi, 3200 psi, 3500 psi, and the temperature conditions were 40°C, 42°C, 45°C, 48°C, and 50°C. This experiment was a factor of two. Due to the central composite design, there are 4 corner points, 4 axial points and 5 repetitions of the central point experiment. At the end of the experiment, a second-order regression analysis of the experimental results was conducted to investigate the significance of the essential oil yield in pressure terms, temperature terms, pressure squared items, temperature squared items, pressure and temperature interaction items, and using Matlab software for mapping. In the figure, a regression line is obtained. Finally, the second-order model is analyzed to obtain the best operating conditions and the best yield in this experiment. Afterwards, the Soxhlet extraction method is used to perform leased-rate extraction of pumpkin seed oil, compared with supercritical The yield of pumpkin seed oil obtained by extraction is consistent. And using a gas chromatography mass spectrometer to analyze whether the fatty acids in the obtained products are also consistent. |
