文化大學機構典藏 CCUR:Item 987654321/39269
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    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/39269


    题名: Two Paralogous Genes Encoding Auxin Efflux Carrier Differentially Expressed in Bitter Gourd (Momordica charantia)
    作者: Li, YL (Li, Yi-Li)
    Lin, YS (Lin, Yun-Shan)
    Huang, PL (Huang, Pung-Ling)
    Do, YY (Do, Yi-Yin)
    贡献者: 生科所
    关键词: auxin transporter
    sequence identity
    phylogenetic analysis
    gene expression
    fruit development
    auxin induction
    日期: 2017-11
    上传时间: 2018-01-11 11:25:13 (UTC+8)
    摘要: The phytohormone auxin regulates various developmental programs in plants, including cell growth, cell division and cell differentiation. The auxin efflux carriers are essential for the auxin transport. To show an involvement of auxin transporters in the coordination of fruit development in bitter gourd, a juicy fruit, we isolated novel cDNAs (referred as McPIN) encoding putative auxin efflux carriers, including McPIN1, McPIN2 (allele of McPIN1) and McPIN3, from developing fruits of bitter gourd. Both McPIN1 and McPIN3 genes possess six exons and five introns. Hydropathy analysis revealed that both polypeptides have two hydrophobic regions with five transmembrane segments and a predominantly hydrophilic core. Phylogenetic analyses revealed that McPIN1 shared the highest homology to the group of Arabidopsis, cucumber and tomato PIN1, while McPIN3 belonged to another group, including Arabidopsis and tomato PIN3 as well as PIN4. This suggests different roles for McPIN1 and McPIN3 in auxin transport involved in the fruit development of bitter gourd. Maximum mRNA levels for both genes were detected in staminate and pistillate flowers. McPIN1 is expressed in a particular period of early fruit development but McPIN3 continues to be expressed until the last stage of fruit ripening. Moreover, these two genes are auxin-inducible and qualified as early auxin-response genes. Their expression patterns suggest that these two auxin transporter genes play a pivotal role in fruit setting and development.
    關聯: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 卷: 18 期: 11 文獻號碼: 2343
    显示于类别:[生物科技研究所 ] 期刊論文

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