本論文中是以固定去氧核醣核酸(DNA)濃度為5%以及不同濃度幾丁聚醣(Chitosan)分別為1%、2%、3%製成多孔材,利用傅立葉可見光吸收光譜儀得知皆含有DNA以及幾丁聚醣之特定官能基。多孔材的孔徑大小會隨著均質機轉速的增加而有所減少,孔隙率會隨著均質機轉速與幾丁聚醣濃度的提高而越來越小,孔隙率都能維持在80%以上。SEM觀察發現多孔材會隨著幾丁聚醣濃度增加,成形性越佳。使用幾丁聚醣,具有環保且生物可分解性之材料,來使去氧核醣核酸不水溶,由實驗結果證明幾丁聚醣可以使去氧核醣核酸達到不水溶化,且不水溶率皆達到85%以上。DNA之混合物由於位於螺旋內部的鹼基有空隙,此大小大約為0.34nm,剛好可以容納苯環化合物,隨著DNA混合物濃度增加,吸收值也隨之上升,是因為孔徑的大小會影響含苯化合物的吸收量,當波長在206.5nm時,可達最高吸附能力。
In this paper, porous materials were prepared with fixed 1%, 2% and 3% of deoxyribonucleic acid (DNA) concentration of 5% and different concentrations of chitosan, and the results were obtained by Fourier visible light absorption spectrometer All contain DNA and chitosan specific functional groups. The pore size of the porous materials decreases with the increase of the rotational speed of the homogenizer. The porosity increases with the increase of the homogenizer speed and chitosan concentration, and the porosity can be maintained above 80% The SEM observation showed that porous materials improved with the increase of chitosan concentration. Using of chitosan, environmentally friendly and biodegradable materials, to make the deoxyribonucleic acid is not water soluble, the experimental results show that chitosan can make the deoxyribonucleic acid to achieve non-water-soluble, and no water solution More than 85%. The DNA mixture has a gap of about 0.34 nm due to the presence of a base in the helix, which can accommodate the benzene ring compound. As the concentration of the DNA mixture increases, the absorption increases as the pore size affects the pore size.The absorption of the compound, when the wavelength at 206.5nm, up to the maximum adsorption capacity.