探討補陽還五湯或天麻鉤藤飲在治療缺血性中風大鼠的影響

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Title:	探討補陽還五湯或天麻鉤藤飲在治療缺血性中風大鼠的影響 The potential effect of Bu Yang Huan Wu Soup or Tianma Gouteng in rat model with ischemic stroke
Authors:	蘇麗宇
Contributors:	生物科技研究所
Keywords:	缺血性中風 ischemic stroke
Date:	2017
Issue Date:	2017-08-10 10:41:39 (UTC+8)
Abstract:	本研究目的在探討利用草藥補陽還五湯及天麻鉤藤飲改善缺血性腦中風大鼠的腦傷情形。中風又稱為「隱形的殺手」，依據衛生福利部國人死因統計顯示，腦血管疾病為國人10大死因的第4位，並且因中風發作時間短暫容易被忽視，而延誤黃金治療時期，但當移除血栓梗塞之後，卻又因為血管血液的再灌流 ( Reperfusion ) 引起一連串的腦傷機制，包括自發性的細胞死亡機制、興奮性毒性和離子不平衡、氧化 ( Oxidative )和亞硝化 ( Nitrosative ) 壓力、類細胞凋亡途徑 ( Apoptotic-like pathway ) 、發炎反應和神經血管相關因子。而有些小中風預後若不做好，也有引發腦中風的可能。本研究使用雄性SD大鼠 ( 週齡約： 6週齡，重量約： 250-300g/BW )，將其兩條總頸動脈 ( Common Carotid Artery, CCA ) 以及大腦的中大腦動脈(Middle Cerebral Artery, MAC)同時綁死結，持續90分鐘後將血管鬆綁，使血液再灌流，以模擬人類急性中風的六小時接著送醫治療後將血栓移除的過程，術後給予不同濃度之補陽環五湯及天麻鉤藤飲。希望能夠藉由先前文獻報導指出的中藥材，探討兩種不同的藥物的療效，並運用在腦中風症狀的改善。實驗結果顯示在管餵食中國的草藥補陽還五湯及天麻鉤藤飲皆有減緩大鼠因中風所引起之中等度腦傷區域。在Triplienyl Tetrazolium Chloride ( TTC ) 染色中發現管餵食補陽還五湯及天麻鉤藤飲兩者的腦傷區域皆有明顯的縮小，隨著劑量的高低效果也不同。在Hematoxylin and Eosin ( H&E ) 染色中可以發現，手術組的大鼠腦切片發炎細胞較多，而給予管餵補陽還五湯及天麻鉤藤飲的大鼠，發炎細胞則是有減少的趨勢，一樣隨著劑量愈高效果也較好。是以前述之實驗結果均顯示有管餵食補陽還五湯及天麻鉤藤飲的缺血性中風大鼠對於腦傷區域部論是腦傷範圍或是發炎反應，皆有中風治療的潛力。 According to the Ministry of Health and Welfare statistics for the cause of people death, cerebrovascular disease is the third of 10 death factors in Taiwan. Stroke episodes are temporarily overlooked, and delayed gold treatment periods. When the thrmbus was removed, it will occur a cascade of blood reperfusion result in the brain injury, such as active cell death mechanisms, excitotoxicity and ionic imbalance, oxidative and nitrosative stress, apoptotic-like pathways, inflammation marker and the neurovascular factors. We hope that we can therapy this "invisible killer" with traditional Chinese medicine. This study is to evaluate the effects in ischemic stroke rat model by Bu Yang Huan Wu Soup and Tianma Gouteng. In this study, two common carotid arteries and the middle cerebral artery (MAC) of the brain were tied knot for 90 mins in SD rats (about 6 weeks old, weighing about 250-300 g/BW). Then blood vessels were released and the blood was reperfusion to simulate the process of removing the thrombus after six hours of human acute stroke. The postoperative rats were given different concentrations of Bu Yang Huan Wu Soup and Tianma Gouteng. Our results demonstrated that rats were treated with Bu Yang Huan Wu Soup and Tianma Gouteng, the cerebral infarction was significant ameliorated. In TTC stain, we found that the brain injury had been reduced and shrink down when the rats were fed with Bu Yang Huan Wu Soup and Tianma Gouteng, similar results also shown in high dose treatment. The brain of rats with cerebral ischemic reperfusion showed inflammation and cell infiltration with hematoxylin and eosin staining, Bu Yang Huan Wu Soup and Tianma Gouteng reduced the inflammation burden both in lower and high dose. In conclusion, our data demonstrated that Bu Yang Huan Wu Soup and Tianma Gouteng can improve the brain injury and inflammation in rats with cerebral ischemic reperfusion.
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