| 摘要: | 本研究目的係探討幾丁聚醣/海藻酸鈉複合材料掺合明膠、及掺合氧化鋁與明膠後，對於動物細胞固定化之培養活性的影響；以作為日後發展桿狀病毒/哺乳動物細胞生物反應器固定化載體用途之評估。在此研究中探討了複合材料在不同酸鹼值下的吸水率、體外酵素降解率、材料混合後之官能基變化以及動物細胞固定化後的活性值。結果顯示，複合材料掺合明膠後吸水率較未掺合明膠平均上升150%；體外酵素降解率平均上升8.33%；細胞活性值平均上升了0.17。選擇活性較高的明膠組來掺合氧化鋁，其結果發現細胞活性值較未掺合氧化鋁者平均上升了0.21；將複合材料製作為疊層化的聚電解質之後發現，製作成三層複合材料的細胞活性值較未分層平均上升了0.15，製作為六層複合材料的細胞活性值較未分層平均上升了0.41。將CHO-K1細胞固定化在掺合氧化鋁的幾丁聚醣/海藻酸鈉/明膠複合薄膜，其平均生長活性為1.979、Vero細胞平均生長活性為1.828、U20S細胞平均生長活性則為1.64。
In this study, we discussed immobilized activity of the material which admix Chitosan , Sodium Alginate, Gelatin and Aluminum oxide. for the animal cells culture. We compared the absorbent rate of the material at the different pH values, the rate of enzymatic degradation in vitro, the functional group changed after the three kinds of materials admixed and the average value which activity of immobilized for the animal cells culture. The result shows, the absorbent rate of the material which admix Gelatin was 1.5 fold higher than the control. The enzymatic degradation rate of the material which admix Gelatin was 8.33% higher than the control in vitro. For the animal cells culture, the average values of immobilized activity which admix Gelatin was 0.17 higher than the control. When the material admix gelatin and aluminum oxide, the average values of immobilized activity was 0.21 higher than the control. The construction of material was layer by layer, the average values of immobilized activity was 0.15% higher than the control. When the construction of material was six layers, the average values of immobilized activity was 0.41 higher than the control. |
