Background: This study investigated the effect of different -6/-3 polyunsaturated fatty acid (PUFA) ratios on dextran sulfate sodium (DSS)-induced changes to small intestinal intraepithelial lymphocyte (IEL) T-cell expression. Methods: Mice were assigned to 3 control and 3 DSS-treated groups and were maintained on a low-fat semipurified diet. One of the control (S) groups and a DSS (DS) group were provided with soybean oil; the other 2 control (H-3 and L-3) groups and 2 other DSS (DH-3 and DL-3) groups were fed either a soybean and fish oil mixture with a -6/-3 ratio of 2:1 or 4:1. After feeding the respective diets for 2 weeks, the DSS groups were given distilled water containing 2% DSS, and the control groups were given distilled water for 5 days. All groups were further provided distilled water 5 days for recovery, and the small intestinal IEL T-cell subset was isolated for analysis. Results: DSS treatment resulted in a lower small intestinal IEL T-cell percentage and higher messenger RNA (mRNA) expressions of Reg III, keratinocyte growth factor (KGF), and complement 5a receptor (C5aR) by IEL T cells. Fish oil administration enhanced the proportion of small intestinal IEL T cells. Compared with the DL-3 group, the DH-3 group had lower Reg III, KGF, and C5aR mRNA expressions and higher expression of peroxisome proliferator-activated receptor (PPAR)- gene by small intestinal IEL T cells. Conclusions: Fish oil diets with a -6/-3 PUFA ratio of 2:1 were more effective than those with a ratio of 4:1 in improving DSS-induced small intestinal injury, and activation of PPAR- in IEL T cells may be associated with resolution of small intestinal inflammation.
關聯:
RNAL OF PARENTERAL AND ENTERAL NUTRITION 卷: 40 期: 3 頁碼: 383-391
