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https://irlib.pccu.edu.tw/handle/987654321/35900
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| Title: | Characterization of promoter activity of the ethylene receptor gene OgERS1 from Oncidesa in transgenic Arabidopsis |
| Authors: | Huang, WF (Huang, W. -F.)
Huang, PL (Huang, P. -L.)
Do, YY (Do, Y. -Y.) |
| Contributors: | 生科所 |
| Keywords: | abscisic acid
abscission layer
actively dividing young tissue
benzylaminopurine
drought;flooding
heat
indole-3-acetic acid
methyl jasmonate
salinity
sugars
wounding |
| Date: | 2016-06 |
| Issue Date: | 2017-04-13 14:48:42 (UTC+8) |
| Abstract: | Physiological changes associated with senescence of flowers and abscission of floral parts in Oncidesa (formerly Oncidium) cv. Gower Ramsey are caused by a plant hormone ethylene which is produced by pollinia cap dislodgment during postharvest handling and transportation. The ethylene receptor gene OgERS1 of Oncidesa has been previously cloned and characterized. To analyze promoter activity of OgERS1, transgenic Arabidopsis thaliana plants were generated to express the -glucuronidase (GUS) reporter gene under the control of 5'-upstream sequence of OgERS1 from Oncidesa. The expression pattern of the OgERS1 promoter at the cellular level was investigated by analysis of GUS activity. This promoter can activate gene expression in both actively dividing young tissues and abscission-related aging tissues. Expression of GUS was detected in the shoot meristem uniquely in 10 to 30 d-old-plants and was found in flower buds, axillary buds, flower stems, and abscission layers during later development. In 2- to 3-week-old transgenic Arabidopsis, exogenous ethylene, glucose, lactose, and maltose enhanced promoter activity implying that crosstalk between sugar and an ethylene receptor may exist. However, indole-3-acetic acid, benzylaminopurine, abscisic acid, heat, wounding, salinity, drought, and flooding slightly suppressed promoter activity. These results demonstrate that the promoter of OgERS1 was developmentally and environmentally regulated, and imply a potential for application of this bi-functional promoter to increase branching or enhanced dwarfing. |
| Relation: | BIOLOGIA PLANTARUM 卷: 60 期: 2 頁碼: 261-268 |
| Appears in Collections: | [Graduate Institute of Biotechnology ] journal articles
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