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| 題名: | Multiple Patterns of Regulation and Overexpression of a Ribonuclease-Like Pathogenesis-Related Protein Gene, OsPR10a, Conferring Disease Resistance in Rice and Arabidopsis |
| 作者: | Huang, LF (Huang, Li-Fen)
Lin, KH (Lin, Kuan-Hung)
He, SL (He, Siou-Luan)
Chen, JL (Chen, Jyh-Lang)
Jiang, JZ (Jiang, Jian-Zhi)
Chen, BH (Chen, Bo-Hong)
Hou, YS (Hou, Yi-Syuan)
Chen, RS (Chen, Ruey-Shyang)
Hong, CY (Hong, Chwan-Yang)
Ho, SL (Ho, Shin-Lon) |
| 貢獻者: | 園生系 |
| 關鍵詞: | LYCOPERSICON-ESCULENTUM CELLS
STARVATION-INDUCED RIBONUCLEASE
DEFENSE-RELATED PROTEINS
BIRCH POLLEN ALLERGEN
CULTURED TOMATO CELLS
AMINO-ACID-SEQUENCE;BET V 1
PHOSPHATE-STARVATION
MOLECULAR CHARACTERIZATION
PROTEOMIC ANALYSIS |
| 日期: | 2016-06 |
| 上傳時間: | 2017-04-13 11:21:51 (UTC+8) |
| 摘要: | An abundant 17 kDa RNase, encoded by OsPR10a (also known as PBZ1), was purified from Pi-starved rice suspension-cultured cells. Biochemical analysis showed that the range of optimal temperature for its RNase activity was 40-70 degrees C and the optimum pH was 5.0. Disulfide bond formation and divalent metal ion Mg2+ were required for the RNase activity. The expression of OsPR10a::GUS in transgenic rice was induced upon phosphate (Pi) starvation, wounding, infection by the pathogen Xanthomonas oryzae pv. oryzae (Xoo), leaf senescence, anther, style, the style-ovary junction, germinating embryo and shoot. We also provide first evidence in whole-plant system, demonstrated that OsPR10a-overexpressing in rice and Arabidopsis conferred significant level of enhanced resistance to infection by the pathogen Xoo and Xanthomona campestris pv. campestris (Xcc), respectively. Transgenic rice and Arabidopsis overexpressing OsPR10a significantly increased the length of primary root under phosphate deficiency (-P-i) condition. These results showed that OsPR10a might play multiple roles in phosphate recycling in phosphate-starved cells and senescing leaves, and could improve resistance to pathogen infection and/or against chewing insect pests. It is possible that Pi acquisition or homeostasis is associated with plant disease resistance. Our findings suggest that gene regulation of OsPR10a could act as a good model system to unravel the mechanisms behind the correlation between Pi starvation and plant-pathogen interactions, and also provides a potential application in crops disease resistance. |
| 關聯: | PLOS ONE 卷: 11 期: 6 文獻號碼: e0156414 |
| 顯示於類別: | [園藝暨生物技術學系] 期刊論文
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