文化大學機構典藏 CCUR:Item 987654321/35884
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 46867/50733 (92%)
造访人次 : 11887238      在线人数 : 748
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于CCUR ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/35884


    题名: Gene structure and expression characteristics of the auxin receptor TIR1 ortholog in Momordica charantia and developmental analysis of its promoter in transgenic plants
    作者: Lin, CK (Lin, Cheng-Ku)
    Lee, NY (Lee, Nien-Ying)
    Huang, PL (Huang, Pung-Ling)
    Do, YY (Do, Yi-Yin)
    贡献者: 生科所
    关键词: Auxin receptor
    Promoter activity
    Gene expression
    Bitter gourd
    Flower development
    日期: 2016-07
    上传时间: 2017-04-13 09:37:17 (UTC+8)
    摘要: It is known that phytohormone auxin affects floral sexual determination in monoecious Cucurbitaceae such as cucumber and melon. TRANSPORT INHIBITOR RESPONSE 1 (TIR1) was first identified in Arabidopsis thaliana as the auxin receptor but few reports have correlated it with fruit initiation and development. As the first step to understanding the regulatory mechanism of auxin in flower sex expression, fruit set, and fruit development in bitter gourd, a TIR1 ortholog named McTIR1, was isolated and characterized in monoecious bitter gourd (Momordica charantia L.). The translation start codon locates in the second exon of McTIR1. Based on phylogenetic analysis, the similarity of deduced amino acid sequence was closer between the McTIR1 and AtTIR1 than between McTIR1 and AUXIN SIGNALING F-BOX (AFB) family. Northern analysis indicated McTIR1 expressed in the early fruit developmental stages of bitter gourd. The McTIR1 mRNA was most abundant in young leaves and stems and lowest in roots of bitter gourd. Expression of McTIR1 was further characterized using transcriptional fusion construct containing beta-glucuronidase reporter gene fused to the gene promoter and 5'-untranslated region of McTIR1. The expression of McTIR1 promoter decreased dramatically during the late stages of pistil development in transgenic tobacco. These results indicate that expression of auxin receptor McTIR1 is associated with the pistil development of unisexual flowers in bitter gourd.
    關聯: JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY 卷: 25 期: 3 頁碼: 253-262
    显示于类别:[生物科技研究所 ] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    index.html0KbHTML214检视/开启


    检视Licence

    在CCUR中所有的数据项都受到原著作权保护.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈