蓮不同部位之乙醇萃取物其抗氧化活性與DNA保護作用之研究

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Title:	蓮不同部位之乙醇萃取物其抗氧化活性與DNA保護作用之研究 Study of Antioxidant Activity and DNA Protection of Ethanolic Extracts for Different Parts of Lotus (Nelumbo nucifera, Gaertn)
Authors:	吳家齊
Contributors:	生物科技研究所
Keywords:	蓮殼蓮蓬蓮子抗氧化能力
Date:	2017
Issue Date:	2017-03-29 14:40:05 (UTC+8)
Abstract:	蓮(Nelumbo nucifera)在古代即被用於醫療用途，其含有許多生物活性物質，包含黃酮類、生物鹼、配糖、三萜類化合物、維生素等，具有個別的療效。在先前各別的研究中證實蓮之不可食部位(蓮殼、蓮蓬)以及可食部位(蓮子)都具有強的抗氧化活性。然而，關於同時測試不同部位之抗氧化活性的相關文獻卻很少，因此本研究的主旨為測試蓮之不同部位(蓮殼、蓮蓬、蓮子)之抗氧化活性與DNA保護效果。將樣品乾燥後使用研磨機研磨成粉，並使用乙醇進行萃取得各部位乙醇萃取物，抗氧化實驗部分將萃取物進行DPPH‧、ABTS‧自由基清除試驗、以及還原力試驗，此外也進行總酚、類黃酮含量的測定以及DNA保護效果試驗。細胞試驗方面則進行毒性試驗及細胞相關抗氧化試驗。在這三種樣品中，蓮殼及蓮蓬具有強的DPPH‧以及ABTS‧自由基清除能力，其IC50顯著低於於蓮子。還原力的部分以EC50作為依據，依數值高低分別為蓮殼＜蓮蓬＜蓮子。總酚及類黃酮含量試驗則是蓮殼＞蓮蓬＞蓮子。DNA保護效果試驗的部分，三種樣品隨著濃度上升皆有保護效果之趨勢。而細胞毒性試驗證實三種樣品皆不具毒性，並且在細胞抗氧化試驗中證實其抗氧化能力。上述結果顯示，以乙醇萃取蓮蓬及蓮殼之萃取物具有良好的抗氧化活性，二種非食用的部位在廢物利用的角度上確實有作為機能性食品的潛力，其潛在的應用值得進一步的研究。 Nelumbo nucifera (lotus) is one of the plants that have been used for its medicinal properties since ancient times. Several bioactive compounds have been derived from these plant parts belonging to different chemical groups, including flavonoids, alkaloids, glycosides, triterpenoid, vitamins etc., which all have their own therapeutic impact. Previous studies show that the inedible parts of lotus (epicarp, seedpod etc.,) and edible parts of lotus (lotus seed) have strong antioxidant activity. However, less information is available about the compare of different parts of lotus simultaneously. Therefore, the present study aimed to evaluate the antioxidant activity and DNA protection of different parts (seedpod, epicarp, and seed) of the lotus. The dry lotus seedpod, epicarp, and seed were crushed using a micro-grinder, then were extracted with ethanol The antioxidant activity was measured by different models, including DPPH．, ABTS．radical scavenging capacity and reducing power. In addition, the extracts were measured the total phenol content, total flavonolic content and examined the DNA protection ability. The lotus epicarp and lotus seedpod had strong DPPH‧ and ABTS‧scavenge activity. The IC50 of the lotus epicarp and lotus seedpod were stronger than the lotus seed. The lotus epicarp had the greatest reducing power. The order of EC50 value in reducing power was lotus epicarp < lotus seedpod < lotus seed. The total phenol and total flavonolic contents distribution in the different part of lotus was as followed: epicarp > lotus seedpod > lotus seed. The DNA protection experiment showed that all samples had DNA protection abilities with increasing concentration. The cytotoxicity test showed that all the sample were not toxic and the cell antioxidant test showed that all the sample had antioxidant activity. These results demonstrated that the ethanolic extracts of lotus epicarp and lotus seedpod have excellent antioxidant activity, indicating their potential applications are worthy of further investigations for salvage.
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