Dimemorfan protects rats against ischemic stroke through activation of sigma-1 receptor-mediated mechanisms by decreasing glutamate accumulation

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Title:	Dimemorfan protects rats against ischemic stroke through activation of sigma-1 receptor-mediated mechanisms by decreasing glutamate accumulation
Authors:	Yuh-Chiang Shen Yea-Hwey Wang Yueh-Ching Chou Kuo-Tong Liou Jiin-Cherng Yen Wen-Yen Wang Jyh-Fei Liao
Contributors:	國術系
Keywords:	cerebral ischemia-reperfusion dimemorfan glutamate inflammation neuroprotection
Date:	2008-01
Issue Date:	2010-06-17 09:33:21 (UTC+8)
Abstract:	Dimemorfan, an antitussive and a sigma-1 (σ<sub>1</sub>) receptor agonist, has been reported to display neuroprotective properties. We set up an animal model of ischemic stroke injury by inducing cerebral ischemia (for 1 h) followed by reperfusion (for 24 h) (CI/R) in rats to examine the protective effects and action mechanisms of dimemorfan against stroke-induced damage. Treatment with dimemorfan (1.0 μg/kg and 10 μg/kg, i.v.) either 15 min before ischemia or at the time of reperfusion, like the putative σ<sub>1</sub> receptor agonist, PRE084 (10 μg/kg, i.v.), ameliorated the size of the infarct zone by 67–72% or 51–52%, respectively, which was reversed by pre-treatment with the selective σ<sub>1</sub> receptor antagonist, BD1047 (20 μg/kg, i.v.). Major pathological mechanisms leading to CI/R injury including excitotoxicity, oxidative/nitrosative stress, inflammation, and apoptosis are all downstream events initiated by excessive accumulation of extracellular glutamate. Dimemorfan treatment (10 μg/kg, i.v., at the time of reperfusion) inhibited the expressions of monocyte chemoattractant protein-1 and interleukin-1β, which occurred in parallel with decreases in neutrophil infiltration, activation of inflammation-related signals (p38 mitogen-activated protein kinase, nuclear factor-κB, and signal transducer and activator of transcription-1), expression of neuronal and inducible nitric oxide synthase, oxidative/nitrosative tissue damage (lipid peroxidation, protein nitrosylation, and 8-hydroxy-guanine formation), and apoptosis in the ipsilateral cortex after CI/R injury. Dimemorfan treatment at the time of reperfusion, although did not prevent an early rise of glutamate level, significantly prevented subsequent glutamate accumulation after reperfusion. This inhibitory effect was lasted for more than 4 h and was reversed by pre-treatment with BD1047. These results suggest that dimemorfan activates the σ<sub>1</sub> receptor to reduce glutamate accumulation and then suppresses initiation of inflammation-related events and signals as well as induction of oxidative and nitrosative stresses, leading to reductions in tissue damage and cell death. In conclusion, our results demonstrate for the first time that dimemorfan exhibits protective effects against ischemic stroke in CI/R rats probably through modulation of σ<sub>1</sub> receptor-dependent signals to prevent subsequent glutamate accumulation and its downstream pathologic events. [ABSTRACT FROM AUTHOR] Copyright of Journal of Neurochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Relation:	Journal of Neurochemistry Vol. 104 Issue 2, P.558-572
Appears in Collections:	[Department of Chinese Martial Arts] journal articles

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