Glutamine Administration After Sublethal Lower Limb Ischemia Reduces Inflammatory Reaction and Offers Organ Protection in Ischemia/Reperfusion Injury

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Title:	Glutamine Administration After Sublethal Lower Limb Ischemia Reduces Inflammatory Reaction and Offers Organ Protection in Ischemia/Reperfusion Injury
Authors:	Shih, YM (Shih, Yao-Ming) Shih, JM (Shih, Juey-Ming) Pai, MH (Pai, Man-Hui) Hou, YC (Hou, Yu-Chen) Yeh, CL (Yeh, Chiu-Li) Yeh, SL (Yeh, Sung-Ling)
Contributors:	營養系
Keywords:	ischemia reperfusion injury hind limb glutamine adhesion molecule inflammatory mediator
Date:	2016-11
Issue Date:	2017-02-16 09:34:34 (UTC+8)
Abstract:	Background: This study investigated the effects of intravenous glutamine (GLN) administration on the expression of adhesion molecules and inflammatory mediators in a mice model of hind limb ischemia/reperfusion (IR) injury. Methods: There were 3 IR groups and 1 normal control (NC) group. The NC group did not undergo the IR procedure. Mice in the IR groups underwent 90 minutes of limb ischemia followed by a variable period of reperfusion. Ischemia was performed by applying a 4.5-oz orthodontic rubber band to the left thigh. Mice in one IR group were sacrificed immediately after reperfusion. The other 2 IR groups were injected once with either 0.75 g GLN/kg body weight (G group) or an equal volume of saline (S group) via tail vein before reperfusion. Mice in the S and G groups were subdivided and sacrificed at 4 or 24 hours after reperfusion. Results: IR enhanced the inflammatory cytokine gene expressions in muscle. Also, plasma interleukin (IL)-6 levels, blood neutrophil percentage, and the adhesion molecule and chemokine receptors expressed by leukocytes were upregulated after reperfusion. The IR-induced muscle inflammatory mediator gene expressions, blood macrophage percentage, and plasma IL-6 concentration had declined at an early or a late phase of reperfusion when GLN was administered. Histologic findings also found that remote lung injury was attenuated during IR insult. Conclusions: A single dose of GLN administration immediately after sublethal lower limb ischemia reduces the inflammatory reaction locally and systemically; this may offer local and distant organ protection in hind limb IR injury.
Relation:	JOURNAL OF PARENTERAL AND ENTERAL NUTRITION 卷:40 期:8 頁碼: 1122-1130
Appears in Collections:	[Department of Food and Nutrition ] journal articles

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