| 摘要: | 青剛櫟（Cyclobalanopsis glauca (Thunb.) Oerst）是富含多酚類化合物之樹種，其中內含之兒茶素（catechins）可增加血漿之抗氧化性，降低罹患心血管疾病的風險，catechins也證實對致癌物質具有抑制作用，特別是可預防皮膚癌和肺癌的發生。本試驗以陽明山之青剛櫟葉片接種購自食品工業研究所二種不同菌株（BCRC15010和BCRC15785）；培養條件中使用α-naphthaleneacetic acid（NAA）與2,4-dichlorophenoxyacetic acid（2,4-D）二種植物生長調節劑。培殖體與BCRC15785菌株進行感染，經45天後，培養於MS培養基（含0.1 mg L^(-1) NAA + 300 mg L^(-1) cefotaxime）中具有較佳之毛狀根（hairy root）誘發率（32.5%）。植物生長調節劑NAA的影響下可誘導出癒合組織和毛狀根，而2,4-D只對癒合組織的誘導有效。研究發現從感染時間1到24小時增加，轉殖率也相對增加。毛狀根、癒合組織和種子苗的根部均有兒茶素的存在，含量濃度分別為4.203、3.413及4.668 mg g^(-1)。
Cyclobalanopsis glauca (Thunb.) Oerst is a tree species with rich polyphenol compounds, including catechins, which increase the oxidation resistance of blood plasma and reduce the risk of cardiovascular disease in humans. Catechin may also have an inhibitory effect on carcinogens, specifically those related to skin and lung cancer. This study investigated hairy root induction in leaves of C. glauca infected with 2 strains of Agrobacterium rhizogenes (BCRC15010 and BCRC15785) under the influence of α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). After 45 d, explants co-cultivated with the BCRC15785 strain in MS medium with 0.1 mg L^(-1) NAA and 300 mg L^(-1) cefotaxime exhibited 32.5% greater efficiency of hairy root induction. Induction of calli and hairy roots was observed under the influence of NAA, while only calli were induced under the influence of 2,4-D. We observed the direct influence of the infection period on the transformation frequency, and it increased as the infection period increased up to 24 h. Extracts from hairy roots, calli, and natural seedling roots revealed the presence of catechin content, and concentrations were 4.203, 3.413, and 4.668 mg g^(-1), respectively, in these 3 analytes. |
