應用普魯士藍測試斑點形成法 (prussian blue test - spot formation method, PBT-SFM)建立動物氰化物中毒之安全簡易速檢技術平臺，將可疑檢體與硫酸置於改良型安全速檢裝置系統，反應釋出氰化物氣體經適度抽氣吸附於含二價鐵(ferrous)濾紙，反應生成亞鐵(ferriferroc)氰化物斑點，由鹽酸脫色展現藍色化合物沈積，經演算分析簡易光學掃瞄濾紙斑點影像的藍色訊號數值，建立半定量檢測回復的回歸校正曲線。初步測試使用標準物劑量5、10、25 與50μg等4種，校正曲線的回歸相關係數為0.9727，標準物溶液的檢測方法極限(method detection limit, MDL)濃度為150μg/L或2.3μM，實際檢體胃內容物和臟器的檢測結果未見陽性。本研究報告實施簡易安全的普魯士藍測試斑點形成法PBT-SFM技術平臺，執行氰化物檢體的半定量安全速檢無需昂貴設備和試劑，檢測靈敏度符合臨床診斷實驗室的氰化物中毒檢體安全速檢需求，適於疑似氰化物中毒的動物案例。
This study aimed to establish rapid detection techniques for cyanide intoxication cases by using the prussian blue test, a spot formation method (PBT-SFM). In practice, cyanide-containing vapor was passed through ferrous filter paper via vacuum system to form ferriferrocyanide spots in situ after being released from specimen with sulfuric acid in an improved reaction chamber.. With subsequent de-coloring process by hydrochloride, visible blue spots were measured by digital image processing and calibration curve regression. Preliminary experimental result at 5, 10, 25, and 50 μg standard doses demonstrated that the minimal detection concentration of standard solution was 150 μg/L or 2.3 μM based on the calibration curve at regression coefficient of 0.9727. Practical detection on collected clinical specimens of stomach content and visceral organs showed negative results. The study established a semi-quantitative test that would not require sophisticated equipment or expensive reagents for rapid detecting cyanide in poisoned specimens by clinical laboratory.
