鴨與鵝之卵巢抑制素α-次單元基因表現與血中抑制素含量之探討

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Title:	鴨與鵝之卵巢抑制素α-次單元基因表現與血中抑制素含量之探討 Gene Expression of Inhibin α-Subunit and Plasma Inhibin Levels in Ducks and Goose
Authors:	王淑音陳陞輝吳兩新
Contributors:	動科系
Keywords:	抑制素鴨鵝基因表現放射性免疫分析產蛋率卵巢
Date:	1999-06
Issue Date:	2016-11-30 14:39:02 (UTC+8)
Abstract:	應用雞之抑制素α-次單元cDNA為探針，以北方雜交方法測出鴨與鵝之卵巢粒性細胞皆表現相同之基因，其mRNA之大小約與雞同(1.7 Kb)。鴨與鵝之其他受測組織包括肝、腎、脾等未能以雞之抑制素α-次單元cDNA測出其表現與否。由表現強度推測鴨之抑制素α- 次單元基因與雞之相同性應高於鵝與雞之間。使用羊之抑制素放射性免疫分析法,測定鴨與鵝血中之抑制素。發現雄鴨之抑制素分泌量低於可偵測之範圍而雌鴨則分泌量隨其個體產蛋性有很大之變異。雄鵝在繁殖季節之抑制素分泌量較非繁殖季低，而雌鵝則相反的在繁殖季節之抑制素分泌量高於非繁殖季。由此可知，繁殖季節之存在與否與禽類之抑制素分泌情形有極大之關聯性，且雄禽與雌禽之抑制素分泌模式亦有相當大之差異。 Chicken inhibin α-subunit cDNA was used as a probe to detect mRNA expression of the same gene in duck and goose ovarian granulosa cells. Results fom Northern blot analysis indicated that the molecular size of the mRNA in duck and goose is similar to that in chicken (1.7 Kb). Degree of inhibin mRNA expression indicated that the homology of the gene between duck and chicken was stronger than that between goose and chicken. Inhibin α-subunit mRNA was not detected in the kidney, spleen or liver. An ovine inhibin radioimmunoassay (RIA) was modified and used in poultry inhibin RIA. Plasma immunoreactive inhibin levels were not detected in male duck, and were not different significantly in female ducks. In seasonal breeder such as goose, the plasma inhibin levels were different between male and female. Concentration of plasma inhibin in male goose was higher in nonbreeding season than that in breeding season. In female goose, plasma inhibin level in high egg producing season was higher than that in low egg producing season and in nonbreeding season. Plasma inhibin levels not only varied between seasonal breeder and non seasonal breeder, but also varied between male and female birds.
Relation:	中國畜牧學會會誌 28:2 民88.06 頁185-194
Appears in Collections:	[Department of Animal Science] journal articles

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