| 摘要: | 本試驗利用羊之抑制素放射性免疫分析系統，建立野禽之抑制素放射性免疫分析法。此系統採用合成之抑制素片段 pI α（1-26）誘導抗體，以 hI α（1-25）作碘化，並以 oI α（1-25）製作標準曲線。結果可測得正值繁殖期之雌性金雞與白鷳血漿中之抑制素，且使用30 ul或 60 ul血漿所得之估算值亦呈劑量反應，此代表羊之抑制素放射性免疫分析系統正確地估算金雞與白鷳血漿中之抑制素含量。此外，無論金雞或白鷳，於繁殖季節，雌禽之抑制素分泌量皆明顯高於雄禽。而於非繁殖季節，雌禽之抑制素分泌量與雄禽則無顯著之差異。 本實驗初步應用已建立之禽類抑制素放射性免疫分析法於金雞、白鷳之繁殖季節變異之探討。日後擴大其應用，必有助於野禽之生殖生理之探討並與馴化家禽相互比較。
An avian inhibin radioimmunoassay was established in this experiment by modifying ovine inhibin radioimmunoassay. The ovine system used pI α(1-26) to induce antibody, and the hI α(1-25) was iodinated as a tracer, and the oI α( 1-25) was used as reference for standard curve. The developed system can detect inhibin from plasma of female Golden pheasant and Silver pheasant during the breeding season and the estimated plasma inhibin levels of 30 ul and 60 ul were parallel to the standard curve, indicating the use of ovine inhibin radioimmunoassay is feasible in application in avian species. Using the validated assay, we found that plasma inhibin levels of both female Golden pheasant and Silver Pheasant were higher than that in males in breeding season. However, there was no significant difference between male and female in nonbreeding season. The avian inhibin radioimmunoassay established in our laboratory has been successfully applied in detecting inhibin from avian species. We expect to expand the use in comparison the reproductive physiology between domestic and wild birds in the future. |
