In this study, chitosan microcapsules containing volatile essential oil were prepared with various deacetylated chitosan wall materials by Orifice Method. The relation of deacetylated degree and viscosity of chitosan; the formation conditions of chitosan microcapsules and sustained release effect of volatile essential oil were investigated. The results indicated that the viscosity of chitosan was decreased with increasing the deacetylated degree of chitosan. The chitosan microcapsule can be obtained at the conditions of deacetylated degree 45%-90% of chitosan. The sustained release effect of volatile essential oil was improved over 100% by microencapsulated with chitosan wall membrane.