細枝柃木之莖部中的化學成分研究

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Title:	細枝柃木之莖部中的化學成分研究 Chemical Constituents From The Stem of Eurya loquaiana
Authors:	駱杰志
Contributors:	化學系應用化學碩士班
Keywords:	細枝柃木 Eurya loquaiana
Date:	2016-06
Issue Date:	2016-08-10 09:35:25 (UTC+8)
Abstract:	細枝柃木(Eurya loquaiana)為山茶科柃木屬植物，主要生長於低到高海拔的山坡林中，分布於台灣全島、安徽、江西、湖北與湖南等地。本研究在探討細枝柃木之化學成分。新鮮的細枝柃木在2014年採集於新北市。先將細枝柃木磨碎，加入甲醇加熱迴流4小時，過濾，取出濾液，依同樣方法重複萃取3次，經合併濾液後利用減壓濃縮取得甲醇提取物，甲醇提取物以乙酸乙酯和水做分配萃取，得到乙酸乙酯層濃縮物與水層濃縮物。將乙酸乙酯層濃縮物經由多次矽膠管柱層析，將其成分分離純化，共到5個純化合物包括-sitosterol (1) 、1-oxo-3-hydroxyolean-18-ene (2)、betulin (3) 、betulinic acid (4)及-sitosterol-3-O--D-glucopyranoside (5)。水層通過離子交換樹酯，以水、50%MeOH、75%MeOH、MeOH沖堤，其中MeOH層再以矽膠與LH-20來分離純化，共得到3個純化合物包括lupeol (6)、betulinaldehyde (7)、3,3’,4-trimethoxyellagic acid-4’-O-glucopyranoside (8)；由水層不溶中分離到apigenin (9)。9個純化合物結構鑑定皆由光譜資料分析(如核磁共振光譜、質譜等)以及經由文獻光譜數據比對。 Eurya loquaiana belongs to Theaceae family Eurya genus, which grows in the low altitudinal to the high altitudinal mountainous areas. E. loquaiana are distributed in Anhui, Jiangxi, Hubei, Hunan, and Taiwan area. In this thesis, the chemical components of E. loquaiana were studied. The fresh E. loquaiana were collected in New Taipei City area in 2014. The stems of E. loquaiana were cut in small species and powdered and then extracted with MeOH under reflux in four times. The combined MeOH extracts were filtered and concentrated. The residues were then partitioned between EtOAc and H2O. The EtOAc layer was concentrated and repeated chromatographyed on silica gel to yield 5 compounds including -sitosterol (1), 1-oxo-3-hydroxyolean-18-ene (2), betulin (3), betulinic acid (4), and sitosterol-3-O--D-glucopyranoside (5). Water layer was column chromatographyed on ion-exchange resin eluting with H2O, 50% MeOH, 75% MeOH, and MeOH, respectively. The MeOH fraction was further chromatographyed on silica gel or Sephadex LH-20 to yield 3 compounds including lupeol (6), betulinaldehyde (7), and 3,3’,4-trimethoxyellagic acid-4’-O-glucopyranoside (8). Water insoluble fraction was purified to obtain apigenin (9). The structures of compound 9 isolated compounds were elucidated on the basis of spectroscopic data analysis.
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