Genotoxic and Cytotoxic Effects of Cyclophosphamide and Three Herbicides in Rana Catesbeiana：Cyclophosphamile及三種殺草劑對牛蛙遺傳毒性與細胞毒性的影響

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题名:	Genotoxic and Cytotoxic Effects of Cyclophosphamide and Three Herbicides in Rana Catesbeiana：Cyclophosphamile及三種殺草劑對牛蛙遺傳毒性與細胞毒性的影響
作者:	陳嘉芬蔡淑宜
贡献者:	生科系
关键词:	牛蛙毒性殺草劑細胞毒性遺傳
日期:	1994-06
上传时间:	2016-07-27 09:50:37 (UTC+8)
摘要:	利用本實驗室發展出的不連續5-Bromodeoxyuridine處理方法，以研究 cyclophosphamide，殺丹 (saturn)，丁基拉草 (butachlor)及甲氧基護古(chlomeoxynil) 對牛蛙(Rana atesbeiana)的遺傳毒性及細胞毒性的影響。Cyclophosphamide為一種已知的抗癌且是致癌劑，saturn,butachlor及chlomeoxynil為臺灣水田最常用的三種殺草劑。Cyclophosphamide在低劑量下，所誘導的姊妹染色分體交換頻率(sister chromatid exchange frequencies,SCE)是與劑量的增加成正比，Saturn及butachlor只在危害動物生命的高劑量下，才會誘導SCE的產生。根據細胞複製指數 (replicative indices)的分析試驗，cyclophosphamide及butachlor只會稍微延緩細胞週期的時間。然而satlirn卻會顯著降低牛蛙骨髓細胞的複製指數。在所測驗的劑量下， chlomeoxynil不會造成SCE顯著增加，但會延緩細胞分裂週期。比較幾個藥品的最低有效劑量,cyclophosphamide所誘導的SCE為saturn的22倍，為butachlor的157倍。本研究結果顯示由本實驗室所發展出來的牛蛙體內SCEs分析方法，可用來偵測環境中化學物質的遺傳毒性，並有助農藥毒性的評估。 A discontinuous 5 - Bromodeoxyuridine administration method developed byour lab, was utilized to detect the genotoxic and cytotoxic effects ofcyclophosphamide, saturn, butachlor, and chlomethoxynil in Rana catesbeiana.Cyclophosphamide, a well known antitumor agent and a carcinogen, yields a dose dependent increase in the SCEs frequency. Saturn and butachlor induced SCEsin bullfrogs only at a dose which affected the viability of these animals. Analysisof the replicative indices showed that cyclophosphamide and butachlor caused aslight cell - cycle delay whereas saturn caused a dose dependent and significantdecrease in replicative index. Chlomethoxynil did not show any significantincrease in SCEs over baseline at the dosage tested. However a delay in cell cyclewas observed by chlomethoxynil. The present study demonstrates that in vivo SCE method in bullfrogs can be used as a monitoring system for the assessment of environmental genotoxic chemicals.
關聯:	華岡理科學報 11 民83.06 頁1-15
显示于类别:	[理學院] 學報-華岡理科學報

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