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    請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/32581


    題名: The Synthesis of N[fed5]-(6-Aminopyridin-2-yl)-2'-deoxycytidine for Recognizing the CG Base Pair at Neutral pH by Oligodeoxyribonucleotide-Directed Triple Helix Formation
    作者: 靳宗玫
    鍾昆元
    陳建志
    林維政
    甘魯生
    貢獻者: 化學系
    關鍵詞: N[fed5]-(6-Aminopyridin-2-yl)-2'-deoxycytidine
    仏C CG base triad;A paper clip type triple helix
    Oligodeoxyribonucleotide
    日期: 1999-10
    上傳時間: 2016-04-13 14:50:47 (UTC+8)
    摘要: The sequence-specific recognition of double-helical DNA by oligodeoxyribonucleotide-directed triple helix (triplex) formation is limited mostly to purine tracts. To interrupt the purine tract in a target sequence, a non-natural deoxyribonucleoside N �� -(6-aminopyridin-2-yl)-2'-deoxycytidine (�k C) was designed to interact with the C base in the CG base pair. The protected phosphoramidite synthon of �k C was synthesized in seven steps and then was incorporated into an oligodeoxyribonucleotide by an automatic DNA synthesizer. Two 22-mers, designated as C2 and P, with a common sequence of 5'-d-T �� CTXT �� CTTCTGTCTCCAGACAG were synthesized in this study. �� C is 5-methyl-2'-deoxycytidine and X is either 2'-deoxycytidine (C) or �k C for C2 and P, respectively. C2 is able to form a paper clip type triplex with one C . CG mismatched base triad in slightly acidic conditions but not at the neutral pH. On the other hand, P forms a stable triplex under both acidic and neutral conditions. This indicates that �k C is able to form a �k C . CG base triad in the triplex. Their physical properties were studied by UV thermal melting experiments and circular dichorism spectroscopy (CD). The thermal melting results imply that the �k C . CG base triad is as stable as the C �均D GC triad at pH 6.0, and �k C helps the triplex formation preferably at neutral to acidic pH. In addition to the hydrogen bonding interaction with the CG base pair, the hydrophobic interaction of �k C may also play an important role in stabilizing the triplex formation of oligodeoxyribonucleotides. In the presence of spermine at either pH 5.0 or pH 6.0, the melting temperature of the third strand of P was elevated about 30 and 21 ℃, respectively. Thus, spermine can enhance the stability of the triple-helical structure.
    關聯: Journal of the Chinese Chemical Society 46:5 民88.10 頁751-758
    顯示於類別:[化學系所] 期刊論文

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