文化大學機構典藏 CCUR:Item 987654321/32175
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    题名: Physiological and proteomic analysis in two wild tomato lines under waterlogging and high temperature stress
    作者: Lin, Hsin-Hung
    Lin, Kuan-Hung
    Syu, Jia-Yu
    Tang, Shun-Yuan
    Lo, Hsiao-Feng
    贡献者: 園生系
    关键词: Ascorbic acids
    Heat tolerance
    Flood tolerance
    Tomato
    Comparative proteomics
    日期: 2016-01
    上传时间: 2016-03-16 15:30:47 (UTC+8)
    摘要: Tomato yields are reduced under waterlogging and high temperature stress condition. Ascorbic acid (ASA) was shown to be involved in tolerance to waterlogging and heat stresses in tomato. Among 44 wild tomato lines treated with waterlogging at 38 A degrees C, L6138 (Solanum peruvianum) showed highest ASA, shoot growth, chlorophyll content and chlorophyll fluorescence in comparison to other tomato lines. Further leaf proteins in L6138 and L0994 under waterlogging at 38 A degrees C for 72 h were analyzed by two-dimensional protein fractionation system. Different protein peaks were analyzed by comparative proteomic analysis and database searching. Fifty protein peaks expressed in response to stress treatment were identified, among which 27 proteins from L0994 and 17 proteins from L6138 were successfully sequenced. Differentially proteins expressed have major functions in protein structure maintenance, metabolism, secretion, translation, biosynthesis, signal transduction, degradation, and photosynthesis under stress. ASA might be played a role in tolerance to waterlogging and heat stress by maintaining RNA transcription, protein structure, and metabolism. In this study, we utilized a physiological and proteomic approach to discover the changes in protein expression profiles of tomatoes in response to heat and flood stresses.
    關聯: JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY 卷: 25 期: 1 頁碼: 87-96
    显示于类别:[園藝暨生物技術學系] 期刊論文

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