文化大學機構典藏 CCUR:Item 987654321/32156
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    题名: Highly sensitive three-dimensional interdigitated microelectrode for microparticle detection using electrical impedance spectroscopy
    作者: Chang, Fu-Yu
    Chen, Ming-Kun
    Wang, Min-Haw
    Jang, Ling-Sheng
    贡献者: 電機系
    关键词: electrical impedance spectroscopy (EIS)
    three-dimensional (3D) interdigitated microelectrode (IME)
    microparticles
    equivalent circuit model (ECM)
    日期: 2016-02-25
    上传时间: 2016-03-11 15:20:59 (UTC+8)
    摘要: Cell impedance analysis is widely used for monitoring biological and medical reactions. In this study, a highly sensitive three-dimensional (3D) interdigitated microelectrode (IME) with a high aspect ratio on a polyimide (PI) flexible substrate was fabricated for microparticle detection (e.g. cell quantity detection) using electroforming and lithography technology. 3D finite element simulations were performed to compare the performance of the 3D IME (in terms of sensitivity and signal-to-noise ratio) to that of a planar IME for particles in the sensing area. Various quantities of particles were captured in Dulbecco's modified Eagle medium and their impedances were measured. With the 3D IME, the particles were arranged in the gap, not on the electrode, avoiding the noise due to particle position. For the maximum particle quantities, the results show that the 3D IME has at least 5-fold higher sensitivity than that of the planar IME. The trends of impedance magnitude and phase due to particle quantity were verified using the equivalent circuit model. The impedance (1269 Omega) of 69 particles was used to estimate the particle quantity (68 particles) with 98.6% accuracy using a parabolic regression curve at 500 kHz.
    關聯: JOURNAL OF PHYSICS D-APPLIED PHYSICS 卷: 49 期: 7 文獻號碼: 075403
    显示于类别:[電機工程系] 期刊論文

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