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| 題名: | Anticolorectal cancer effects and pharmacokinetic application of 2, 2-Bis [4-(4-amino-3-hydroxyphenoxy) phenyl] adamantane |
| 作者: | Yang, Po-Sheng
Wang, Jane-Jen
Tsai, Tung-Hu
Wang, Yea-Hwey
Jan, Woan-Ching
Cheng, Shih-Ping
Chi, Chin-Wen
Hsu, Yi-Chiung |
| 貢獻者: | 生科系 |
| 關鍵詞: | VIVO GROWTH-INHIBITION
HUMAN COLON-CANCER
IN-VITRO
G(1) ARREST
ERK PATHWAY
CELL LINES
DERIVATIVES
P21(CIP/WAF1)
ACTIVATION
DIAMANTANE |
| 日期: | 2015 |
| 上傳時間: | 2016-03-10 15:52:59 (UTC+8) |
| 摘要: | 2, 2-Bis (4-(4-amino-3-hydroxyphenoxy) phenyl) adamantane (DPA) induced growth inhibition in human cancer cells using the national cancer institute (NCI) anticancer drug screen. In our previous study, we demonstrated that DPA exerted growth inhibitory activities in the three human colon cancer cell lines (Colo 205, HT-29, and HCT-15). To identify the detailed mechanism, we examined the functional importance of p21 and p53 in DPA-induced anticancer effect. We used three isogenic colon cancer cell lines, HCT-116, HCT-116 p53(-/-), and HCT-116 p21(-/-), to evaluate the roles of p21 and p53 in the in vitro anticancer effects of DPA. DPA dose-dependently inhibited cell growth, cell migration and increased cell cycle at the G(0)/G(1) phase in HCT116 cells but not in p21(-/-) and p53(-/-) isogenic HCT-116 cells. Additionally, Western blot showed that DPA treatment induced the p21, p53, and cyclin-E protein expressions in HCT-116 cells. The p21 associated cell cycle regulatory protein such as cyclin D, CDK4, and pRb were decreased after DPA treatment in HCT-116 cells. DPA decreased cell migration in HCT-116 and HCT-116 p53(-/-) but not in HCT-116 p21(-/-) cells. We observed the up-regulation of E-cadherin, p-p38,and p-Erk in DPA-treated HCT-116 group but not in HCT-116 p21(-/-) and HCT-116 p53(-/-) groups. We assumed that p21 was required for DPA-induced anti-colon cancer effect through the Erk and p38 pathway leading to cell cycle arrest and inhibition of cell motility. Mean (+/- SE) pharmacokinetic parameters of the DPA were as follows: AUC = 64.44 +/- 8.41, Cmax = 1.56 +/- 0.48 and t(1/2) = 113.92 +/- 58.19. The pharmacokinetic data suggest DPA can be applied to further clinical study. This is the first pharmacokinetic study of DPA, and indicated that anti-proliferation and the cell mobility inhibition effects of DPA in HCT116 WT cells may result from the induction of p21 through activation of ERK and p38 pathway. |
| 關聯: | INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 卷: 8 期: 9 頁碼: 14805-14815 |
| 顯示於類別: | [生命科學系] 期刊論文
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