Overexpression of the genes coding ascorbate peroxidase from Brassica campestris enhances heat tolerance in transgenic Arabidopsis thaliana

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题名:	Overexpression of the genes coding ascorbate peroxidase from Brassica campestris enhances heat tolerance in transgenic Arabidopsis thaliana
作者:	Chiang, C.M. Chien, H.L. Chen, L.F.O. Hsiung, T.C. Chiang, M.C. Chen, S.P. Lin, K.H.
贡献者:	生科所
关键词:	Agrobacterium-mediated transformation Chinese cabbage chlorophyll hydrogen peroxide malondialdehyde
日期:	2015-06
上传时间:	2016-03-04 16:24:54 (UTC+8)
摘要:	Previously, the ascorbate peroxidase (APX1) activity and gene expression in Chinese cabbage (Brassica campestris, Bc) heat-tolerant cv. ASVEG2 were found to be significantly higher than in heat-sensitive cv. RN720 under a heat stress. Furthermore, BcAPX2 and BcAPX3, isoforms of BcAPX1, were cloned in this study. Our objective was to transfer BcAPX cDNA under the control of the ubiquitin promoter to Arabidopsis via Agrobacterium tumefaciens strain GV3101. We found that BcAPX genes were overexpressed in transgenic Arabidopsis, and the expression of APX, and the APX activity in transgenic lines were higher than in non-transgenic (NT) plants under high temperatures. The chlorophyll content and the germination rate were significantly higher, and the malondialdehyde content was lower in BcAPX1-3, 2-1, and 3-5 lines subjected to the heat-stress treatment than those in the NT plants. Compared to the NT plants, a lower heat-induced H2O2 accumulation was detected by diaminobenzidine staining in leaves of the transgenic lines with a high APX activity indicating that the overexpression of BcAPX in Arabidopsis could enhance heat tolerance by eliminating H2O2.
關聯:	BIOLOGIA PLANTARUM 卷: 59 期: 2 頁碼: 305-315
显示于类别:	[生物科技研究所 ] 期刊論文

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