樟芝超臨界流體萃取物的保健活性分析

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Title:	樟芝超臨界流體萃取物的保健活性分析 Analysis of Nutraceutical Activity for The Extracts Prepared by Supercritical Fluid Extraction from Antrodia cinnamomea
Authors:	陳亮中
Contributors:	生物科技研究所
Keywords:	牛樟芝超臨界流體 C型肝炎 Antrodia Cinnamomea Supercritical Fluid Extraction Hepatitis C Virus
Date:	2015
Issue Date:	2016-01-22
Abstract:	台灣特有種牛樟芝的被開發議題，所觸動的不獨在其實際的醫療效果，更擴大含攝生物多樣性與原始民族用藥經驗等以下的研究領域，這些研究領域的發展與世界衛生組織發表的傳統醫學策略(WHO Traditional Medicine Strategy)同一脈絡。而從1989年被發現以來，Hepatitis C virus (HCV) 已經成為世界上主要的慢性肝臟疾病之一並且有高發病與死亡機率，也導致肝癌與肝硬化。長期受到HCV感染的患者容易轉變成肝癌，而現今有研究也指出樟芝也在治療HCV是上也是有效果的。所以在這篇研究裡面有兩個主要的目的，找到在穀類上最合適的樟芝培養條件。以及試驗樟芝的超臨界萃取物對HCV NS3/4A 蛋白酶的抑制作用。結果表明，無論是在蛋白質含量固定的組別內或是碳水化合物含量固定的組別內，以燕麥作為穀類培養基皆呈現最佳的生長情形。在對HCV NS3/4A 蛋白酶的抑制作用試驗中，我們得知經固態培養的樟芝經過超臨界萃取後，能對在濃度為1.2916 μg/ml的時候，達到IC50的抑制效果。 Anrtodia cinnamomea, a medicinal fungus, grows on the inner surface of decayed Cinnamomum kanehirae in Taiwan ,with an annual market of over $100 million (US) in Taiwan. It is used to treat toxicities caused by food, alcohol, and drugs as well as diarrhea, abdominal pain, hypertension, itchy skin, and tumors. Since its discovery in 1989, hepatitis C virus (HCV) has been recognised as a major cause of chronic liver disease worldwide.Approximately 85% of persons infected with HCV develop chronic hepatitis and may lead to hepatocellular carcinoma. Antrodia cinnamomea is also a highly valued folk medicine used for liver cancer, often caused by long tern infection of heptitis C virus.There are two purposes in this study. To find out the best grain medium for growing Antrodia Cinnamomea, and to examing the ability of Antrodia Cinnamomea extracts from supercritical fluid CO2 extraction against HCV NS3/4A protease. The results showed that the best grain medium for Antrodia cinnamomea was oat medium whatever carbohydrate or protein content was fixed. In the inhibitory activity analysis, the SCF CO2 extraction of solid medium culture Antrodia cinnamomea possessed good inhibit activity against HCV NS3/4A protease with IC50 of 1.2916 μg/ml.
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