The capsid protein VP1 of chicken anemia virus (CAV) is an immunogen usually to be used when developing the diagnostic kits and subunit vaccines. In this study, a codon-optimized VP1 gene of CAV was used to express VP1 in Escherichia coli. To establish the best culture medium for VP1 production, the modified super optimal broth with catabolic repressor (mSOC) medium was selected, out of eight commonly used media, as the basal medium for optimization. The 2(4) factorial experimental results demonstrated that only two constituents of mSOC, peptone and mixed ionic solution, had a significant effect on VP1 production. After a steepest ascent experiment, a rotatable central composite design (CCD) approach was applied to optimize these variables with respect to VP1 production. When the concentrations of peptone (1.79%) and mixed ionic solution (0.27%) predicted by the CCD approach were used with mSOC, a maximum VP1 yield of 138.68 mu gmL(-1) was achieved. The use of this optimized mSOC medium resulted in a 1.84-fold increase in VP1 protein production compared to LB medium. The optimized mSOC identified herein has potential to be used in the future for the industrial large-scaled VP1 production to diagnostic or a subunit vaccine application. (c) 2014 Curtin University of Technology and John Wiley & Sons, Ltd.
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ASIA-PACIFIC JOURNAL OF CHEMICAL ENGINEERING 卷: 10 期: 1 頁碼: 96-104
