文化大學機構典藏 CCUR:Item 987654321/30775
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    题名: Comparative proteomic analysis of cauliflower under high temperature and flooding stresses
    作者: Lin, K.H.
    Chen, L.F.O.
    Li, S.D.
    Lo, H.F.
    贡献者: Grad Inst Biotechnol
    关键词: Comparative proteomics
    Cauliflower
    Heat and flood tolerance
    Differentially expressed proteins
    日期: 2015-02
    上传时间: 2015-10-30 14:34:15 (UTC+8)
    摘要: High-temperature and waterlogging are major abiotic stresses that affect the yield and quality of cauliflower. Cauliflower cultivars 'H41' and 'H69' are tolerant to high temperature and flooding, respectively; however, 'H71' is sensitive to both stresses. The objectives of this study were to identify the proteins that were differentially regulated and the physiological changes that occurred during different time periods in 'H41', 'H69', and 'H71' when responding to treatments of flooding, 40 C, and both stresses combined. Changes in the leaf proteome were analyzed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) and identified by Mascot peptide mass fingerprint (PMF) and database searching. Stress treatments caused significant reductions in electrolyte leakage, chlorophyll fluorescence Fv/Fm, chlorophyll content, and water potential as stress times were prolonged. By the comparative proteomic analysis, 85 protein peaks that were differentially expressed in response to combination treatments at 0, 6, and 24h, 69 (33 in 'H41', 29 in 'H69', and 9 in 'H71') were identified, of which were cultivar specific. Differentially regulated proteins predominantly functioned in photosynthesis and to a lesser extent in energy metabolism, cellular homeostasis, transcription and translation, signal transduction, and protein biosynthesis. This is the first report that utilizes proteomics to discover changes in the protein expression profile of cauliflower in response to heat and flooding. (C) 2014 Elsevier B.V. All rights reserved.
    關聯: SCIENTIA HORTICULTURAE 卷: 183 頁碼: 118-129
    显示于类别:[生物科技研究所 ] 期刊論文

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