文化大學機構典藏 CCUR:Item 987654321/30583
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    题名: Proteomic analysis of broccoli (Brassica oleracea) under high temperature and waterlogging stresses
    作者: Lin, Hsin-Hung
    Lin, Kuan-Hung
    Chen, Su-Ching
    Shen, Yu-Hsing
    Lo, Hsiao-Feng
    贡献者: Dept Hort & Biotechnol
    关键词: Ribulose bisphosphate carboxylase
    Broccoli
    Heat and waterlogging stresses
    Differentially expressed proteins
    日期: 2015-07
    上传时间: 2015-10-20 16:26:37 (UTC+8)
    摘要: Background: The production of broccoli (Brassica oleracea) is largely reduced by waterlogging and high temperature stresses. Heat-tolerant and heat-susceptible broccoli cultivars TSS-AVRDC-2 and B-75, respectively, were used for physiological and proteomic analyses. The objective of this study was to identify TSS-AVRDC-2 and B-75 proteins differentially regulated at different time periods in response to waterlogging at 40 degrees C for three days.

    Results: TSS-AVRDC-2 exhibited significantly higher chlorophyll content, lower stomatal conductance, and better H2O2 scavenging under stress in comparison to B-75. Two-dimensional liquid phase fractionation analyses revealed that Rubisco proteins in both varieties were regulated under stressing treatments, and that TSS-AVRDC-2 had higher levels of both Rubisco large and small subunit transcripts than B-75 when subjected to high temperature and/or waterlogging.

    Conclusions: This report utilizes physiological and proteomic approaches to discover changes in the protein expression profiles of broccoli in response to heat and waterlogging stresses. Higher levels of Rubisco proteins in TSS-AVRDC-2 could lead to increased carbon fixation efficiency to provide sufficient energy to enable stress tolerance under waterlogging at 40 degrees C.
    關聯: BOTANICAL STUDIES 卷: 56 文獻號碼: 18
    显示于类别:[園藝暨生物技術學系] 期刊論文

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