| Title: | Activity staining of isocitrate lyase after electrophoresis on either native or sodium dodecyl sulfate polyacrylamide gels |
| Authors: | Hou WC
Chen HJ
Lin YH
Chen YC
Yang LL
Lee MH |
| Contributors: | 園藝系 |
| Keywords: | activity staining polyacrylamide gel electrophoresis
isocitrate lyase
native sulfate-polyacrylamide gel electrophoresis sodium dodecyl |
| Date: | 2001 |
| Issue Date: | 2009-12-11 11:27:25 (UTC+8) |
| Abstract: | Isocitrate was cleaved into succinate and glyoxylate by isocitrate lyase (ICL) in the glyoxylate cycle. We used lactate dehydrogenase as an ancillary enzyme to further metabolize the glyoxylate to glycolate in the presence of NADH. 3-(4,5-Dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) and 2,6-dichlorophenol-indolphenol (DCPIP) were used in the coupling reactions for detecting ICL activity after electrophoresis on either native or sodium dodecyl sulfate (SDS) polyacrylamide gels. This fast and sensitive method can be used in the process of ICL enzyme purification and characterization. |
| Relation: | ELECTROPHORESIS Volume: 22 Issue: 13 Pages: 2653-2655 |
| Appears in Collections: | [Department of Horticulture] journal articles
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