Formation of DNA triple helix containing N-4-(6-aminopyridin-2-yl)-2 '-deoxycytidine

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Title:	Formation of DNA triple helix containing N-4-(6-aminopyridin-2-yl)-2 '-deoxycytidine
Authors:	Chin TM;Tseng MH;Chung KY;Hung FS;Lin SB;Kan LS
Contributors:	化學系
Date:	2001
Issue Date:	2009-12-11 10:53:40 (UTC+8)
Abstract:	A cytidinyl derivative, N-4-(6-aminopyridin-2-yl)-2'-deoxycytidine (PC), could interact with a CG base pair to support the triple-helix (triplex) formation of oligodeoxyribonucleotides. Characteristics of PC in the formation of both intramolecular triplex, i.e., a "paper clip type" triplex (PCT) and intermolecular triplex. i.e., a "linear type" triplex (LT) was monitored by optical methods and isothermal titration calorimetric measurements. Experimental results revealed that the LT with PC.CG internally was independent of the solution pH. Only single substitution of PC, situated internally but not terminally, facilitated the PCT formation by the UV thermal melting study at the neutral pH, However, the best stabilization of the PCT in acidic conditions occurred when PC at the end of the triplex rather than internally. In addition, an LT, but not a PCT, containing an alternating (PCTCTC)-C-p-C-p sequence, could be formed in the conditions of 20 mM MgCl2 and/or 5 mM spermine. Thus, the presence of several nucleotides of PC in proximity along the Hoogsteen strand may lead to structural distortion such that the more flexible LT with multiple substitutions is formed in favor of the more rigid PCT.
Relation:	JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS Volume: 19 Issue: 3 Pages: 543-553
Appears in Collections:	[Department of Chemistry & Graduate Institute of Applied Chemistry ] journal articles

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