Activity staining of glutathione peroxidase after electrophoresis on native and sodium dodecyl sulfate polyacrylamide gels

文化大學機構典藏 CCUR > 環境規劃暨生物資源學院 > 農學院 > 園藝暨生物技術學系 > 期刊論文 > Item 987654321/2984

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题名:	Activity staining of glutathione peroxidase after electrophoresis on native and sodium dodecyl sulfate polyacrylamide gels
作者:	Lin CL Chen HJ Hou WC
贡献者:	園藝系
关键词:	activity staining glutathione peroxidase native polyacrylamide gel electrophoresis sodium dodecyl sulfate-polyacrylamide gel electrophoresis
日期:	2002
上传时间:	2009-12-11 09:48:30 (UTC+8)
摘要:	Glutathione peroxidase (GSH-Px), from commercial bovine erythrocytes or ammonium sulfate fractionations (30-45%, 45-60%, 60-75% and 75-90% saturations) of ginger rhizome, was detected on polyacrylamide gels after native polyacrylamide gel electrophoresis (PAGE) or sodium dodecyl sulfate (SDS)-PAGE. The gel was submerged in a 50 mm Tris-HCl buffer (pH 7.9) containing 13 mm glutathione and 0.004% hydrogen peroxide with gentle shaking for 10-20 min. The GSH-Px activity was stained with a solution containing 1.2 mm 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 1.6 mm phenazine methosulfate (PMS) for 10 min. The clear zone of GSH-Px activity on a purple background was found in both native and SDS-PAGE gels. This fast and sensitive method can be used in the process of enzyme purification and characterization of mammalian or plant cells.
關聯:	ELECTROPHORESIS Volume: 23 Issue: 4 Pages: 513-516
显示于类别:	[園藝暨生物技術學系] 期刊論文

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