生質酒精可由澱粉類或纖維素成份原料分解成葡萄糖單位再進行發酵產生生質酒精，本實驗是以芒草為原料，芒草為禾本草本植物帶有大量的木質纖維素可做為生質酒精之基底，生長快速且容易生長做為生產酒精之原料極佳。
實驗所使用前處理是以硫酸鹽蒸煮法，先以NaOH及Na2S以硫化度25%，活性鹼20%配置蒸煮液，再取芒草粉及芒草片以三種不同固液比蒸煮，至於高溫爐中以150-165° C 蒸煮 3小時，將芒草中木質素脫去藉以分解出纖維素及半纖維素，破壞高分子結構形成小單位纖維素及木質素鏈結。
蒸煮後進行殘渣過濾再加入纖維水解酵素及纖維糖化酵素，置於恆溫水槽中以40°C放置72小時進行水解糖化反應生成葡萄糖，每24小時測量其糖度變化並記錄，最後再加入酵母水溶液，進行發酵反應，進而製成生質酒精。測量蒸煮前後之pH值及最佳溫度、進行水解糖化反應時之糖度變化、發酵後所生成生質酒精含量及生成物比例，最後在比較兩種鹼法蒸煮差異。
Bio-ethanol can prepare by decomposing starch or cellulose into glucose units ingredients then fermented to produce bio-ethanol . The experiment is miscanthus as ethanol material, due to the nature of matter Wo miscanthus as lignin and cellulose with a lot of living to do the quality of the base alcohol , and a lot of other fast-growing , as the material for ethanol production is excellent choise.
Kraft pretreatment cooking method is used in this experiment . First , NaOH and Na2S 25% sulfidity , 20% active alkalinity to configure the cooking liquid , then take miscanthus powder and pieces to liquid three different ratio of adding cooking liquid cooked to 150-165 ℃ for 3 hours, cellulose and lignin cooking Miscanthus decomposition, destruction of the polymer structure of cellulose and lignin formation of small units link .
Added fiber and fiber hydrolysing enzyme saccharification enzymes , placed in a constant temperature water bath to 40 °C for 72 hours saccharification reaction of glucose every 24 hours to measure its sugar content and record the change , and finally the yeast was added an aqueous solution of the fermentation reaction , turn into bio-ethanol . Brix measurements of changes in saccharification reaction , and measuring the ratio of the aqueous solution of glucose sugar content . Finally, measuring the biomass produced by the fermentation of alcohol and the resultant ratio.
