文化大學機構典藏 CCUR:Item 987654321/2969
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    题名: Genotyping and assessment of genetic relationships in elite polycross breeding cultivars of sweet potato in Taiwan based on SAMPL polymorphisms
    作者: Tseng Y.T.
    Lo H.F.
    Hwang S.Y.
    贡献者: 園藝系
    关键词: genetic relationships
    polycross
    SAMPL
    sweet potato
    日期: 2002
    上传时间: 2009-12-07 11:48:29 (UTC+8)
    摘要: Selective amplification of microsatellite polymorphic loci (SAMPL) markers was used to analyze the genetic relationships between 22 elite cultivars of sweet potato [Ipomoea batatas (L.) Lam.] used in polycross breeding in Taiwan. These elite cultivars included varieties introduced from China and Japan and landraces from Taiwan as well as cultivars derived either from hybrid or polycross breeding programs. Among 12 SAMPL primer pairs tested, 7 amplified 19 loci while the other 5 SAMPL primer pairs gave ambiguous banding profiles. Fifty-five alleles were amplified in total. The unweighted pair group method with arithmetic mean (UPGMA) and principal coordinate analysis (PCA) of SAMPL data suggest that Taiwan landraces are distantly related to Chinese and Japanese cultivars after many years of independent selection and may have originated from Java and Brahman. Polycross derived cultivars are closely related to different landraces. Employment of SAMPL markers is efficient compared to other molecular marker methods, such as random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR). in assessing the genetic relationships of sweet potato cultivars used in the polycross breeding program in Taiwan.
    關聯: BOTANICAL BULLETIN OF ACADEMIA SINICA Volume: 43 Issue: 2 Pages: 99-105
    显示于类别:[園藝暨生物技術學系] 期刊論文

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