Linoleic acid isomerase activity in enzyme extracts from Lactobacillus acidophilus and Propionibacterium freudenreichii ssp shermanii

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题名:	Linoleic acid isomerase activity in enzyme extracts from Lactobacillus acidophilus and Propionibacterium freudenreichii ssp shermanii
作者:	Lin T.Y. Lin C.W. Wang Y.J.
贡献者:	動科系
关键词:	conjugated linoleic acid isomerase Lactobacillus acidophilus Propionibacterium freudenreichii ssp shermanii
日期:	2002
上传时间:	2009-12-07 11:09:20 (UTC+8)
摘要:	Enzyme extracts from Lactobacillus acidophilus (CCRC14079) and Propionibacterium freudenreichii ssp. shermanii (CCRC11076) were reacted with linoleic acid at 50 degreesC for 10 min at pH 5, 6, 7, and 8, and the levels of conjugated linoleic acid (CLA) formation were determined by high performance liquid chromatography. CLA formations were observed in all the reactions catalyzed by the retentates using ultrafiltration membranes with 100 kDa nominal molecular weight cutoff, indicating the presence of the activity of linoleic acid isomerase with nominal molecular weight higher than 100 kDa in the retentates from two cultures. More CLA was formed at pH 5 of L. acidophilus treatment and t10c12, c11t13, and c9t11 CLA were 3 major CLA isomers produced. Results demonstrate a potential for CLA production through linoleic acid isomerase.
關聯:	JOURNAL OF FOOD SCIENCE Volume: 67 Issue: 4 Pages: 1502-1505
显示于类别:	[動物科學系 ] 期刊論文

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