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| 題名: | Frequent Co-Expression of miRNA-5p and-3p Species and Cross-Targeting in Induced Pluripotent Stem Cells |
| 作者: | Huang, Chiu-Jung
Choo, Kong Bung
Sugii, Shigeki
Wee, Kenneth
Cheong, Soon Keng
Kamarul, Tunku |
| 貢獻者: | 動物科學系 |
| 關鍵詞: | Induced pluripotent stem cells
reprogramming
miRNA-5p/3p species
cell cycle control |
| 日期: | 2014 |
| 上傳時間: | 2015-01-27 14:53:52 (UTC+8) |
| 摘要: | Background: A miRNA precursor generally gives rise to one major miRNA species derived from the 5' arm, and are called miRNA-5p. However, more recent studies have shown co-expression of miRNA-5p and -3p, albeit in different concentrations, in cancer cells targeting different sets of transcripts. Co-expression and regulation of the -5p and -3p miRNA species in stem cells, particularly in the reprogramming process, have not been studied.
Methods: In this work, we investigated co-expression and regulation of miRNA-5p and -3p species in human induced pluripotent stem cells (iPSCs), mesenchymal stem cells (MSCs) and embryonic stem cells (ESC) using a nanoliter-scale real-time PCR microarray platform that included 1,036 miRNAs.
Results: In comparing iPSC and ESC, only 32 miRNAs were found to be differentially expressed, in agreement of the ESC-like nature of iPSC. In the analysis of reprogramming process in iPSCs, 261 miRNAs were found to be differentially expressed compared with the parental MSC and pre-adipose tissue, indicating significant miRNA alternations in the reprogramming process. In iPSC reprogrammed from MSC, there were 88 miRNAs (33.7%), or 44 co-expressed 5p/3p pairs, clearly indicating frequent co-expression of both miRNA species on reprogramming. Of these, 40 pairs were either co-up-or co-downregulated indicating concerted 5p/3p regulation. The 5p/3p species of only 4 pairs were regulated in reverse directions. Furthermore, some 5p/3p species of the same miRNAs were found to target the same transcript and the same miRNA may cross-target different transcripts of proteins of the G1/S transition of the cell cycle; 5p/3p co-targeting was confirmed in stem-loop RT-PCR.
Conclusion: The observed cross-and co-regulation by paired miRNA species suggests a fail-proof scheme of miRNA regulation in iPSC, which may be important to iPSC pluripotency. |
| 關聯: | INTERNATIONAL JOURNAL OF MEDICAL SCIENCES 卷: 11 期: 8 頁碼: 824-833 |
| 顯示於類別: | [動物科學系 ] 期刊論文
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