文化大學機構典藏 CCUR:Item 987654321/29189
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 47225/51091 (92%)
造访人次 : 14006738      在线人数 : 231
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于CCUR ‧ 管理 到手机版


    請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/29189


    題名: Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Prostate Cancer Cell Migration and Invasion Induced by Tumor-Associated Macrophages
    作者: Li, Cheng-Chung
    Hou, Yu-Chen
    Yeh, Chiu-Li
    Yeh, Sung-Ling
    貢獻者: 食品暨保健營養學系
    關鍵詞: ACTIVATED-RECEPTOR-GAMMA
    NF-KAPPA-B
    N-3 FATTY-ACIDS
    BREAST-CANCER
    PPAR-GAMMA
    MATRIX METALLOPROTEINASES
    IN-VIVO
    PROGRESSION
    PATHWAY
    GROWTH
    日期: 2014-06-12
    上傳時間: 2015-01-26 09:48:37 (UTC+8)
    摘要: Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are the major n-3 polyunsaturated fatty acids (PUFAs) in fish oil that decrease the risk of prostate cancer. Tumor-associated macrophages (TAMs) are the main leukocytes of intratumoral infiltration, and increased TAMs correlates with poor prostate cancer prognosis. However, the mechanism of n-3 PUFAs on prostate cancer cell progression induced by TAMs is not well understood. In this study, we investigated the effects of EPA and DHA on modulating of migration and invasion of prostate cancer cells induced by TAMs-like M2-type macrophages. PC-3 prostate cancer cells were pretreated with EPA, DHA, or the peroxisome proliferator-activated receptor (PPAR)-gamma antagonist, GW9662, before exposure to conditioned medium (CM). CM was derived from M2-polarized THP-1 macrophages. The migratory and invasive abilities of PC-3 cells were evaluated using a coculture system of M2-type macrophages and PC-3 cells. EPA/DHA administration decreased migration and invasion of PC-3 cells. The PPAR-gamma DNA-binding activity and cytosolic inhibitory factor kappa B alpha (I kappa B alpha) protein expression increased while the nuclear factor (NF)-kappa B p65 transcriptional activity and nuclear NF-kappa B p65 protein level decreased in PC-3 cells incubated with CM in the presence of EPA/DHA. Further, EPA/DHA downregulated mRNA expressions of matrix metalloproteinase-9, cyclooxygenase-2, vascular endothelial growth factor, and macrophage colony-stimulating factor. Pretreatment with GW9662 abolished the favorable effects of EPA/DHA on PC-3 cells. These results indicate that EPA/DHA administration reduced migration, invasion and macrophage chemotaxis of PC-3 cells induced by TAM-like M2-type macrophages, which may partly be explained by activation of PPAR-gamma and decreased NF-kappa B p65 transcriptional activity.
    關聯: PLOS ONE 卷: 9 期: 6 文獻號碼: e99630
    顯示於類別:[保健營養學系 ] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML509檢視/開啟


    檢視Licence

    在CCUR中所有的資料項目都受到原著作權保護.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback