Expression of the broccoli catalase gene (BoCAT) enhances heat tolerance in transgenic Arabidopsis

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Title:	Expression of the broccoli catalase gene (BoCAT) enhances heat tolerance in transgenic Arabidopsis
Authors:	Chiang, Chih-Ming Chen, Shi-Peng Chen, Long-Fang O. Chiang, Ming-Chang Chien, Hao-Lun Lin, Kuan-Hung
Contributors:	生物科技研究所
Keywords:	Agrobacterium-mediated transformation Broccoli Catalase Heat tolerance Transgenic Arabidopsis
Date:	2014-07
Issue Date:	2015-01-26
Abstract:	The objective of this study was to transfer catalase gene (CAT1 and CAT2) complementary (c)DNAs under the control of a ubiquitin promoter into Arabidopsis via Agrobacterium-mediated transformation. A real-time polymerase chain reaction analysis demonstrated that both the BoCAT1 and BoCAT2 genes were overexpressed in transgenic Arabidopsis thaliana (At). The activity of CAT in the AtCAT2-2 transgenic line was 6-fold higher than that of the non-transgenic plant under heat stress, and the CAT amount in the AtCAT2-2 line also highly accumulated according to a Western blot analysis. Compared to non-transgenic Arabidopsis plants, a lower level of heat-induced H2O2 accumulation was detected by diaminobenzidine staining in leaves of transgenic plants with a high level of CAT activity, indicating that overexpression of BoCAT in Arabidopsis could enhance the heat tolerance by eliminating H2O2. This is the first report suggesting that CAT-encoding gene expression in Arabidopsis is regulated by heat stress.
Relation:	JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY 卷: 23 頁碼: 266-277
Appears in Collections:	[Graduate Institute of Biotechnology ] journal articles

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