文化大學機構典藏 CCUR:Item 987654321/2912
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 46867/50733 (92%)
Visitors : 11891311      Online Users : 603
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version


    Please use this identifier to cite or link to this item: https://irlib.pccu.edu.tw/handle/987654321/2912


    Title: Piezoelectric crystal immunosensor for the detection of staphylococcal enterotoxin B
    Authors: Lin HC
    Tsai WC
    Contributors: 生科所
    Keywords: piezoelectric quartz crystal
    immunosensor
    label-free immunoassay
    staphylococcal enterotoxin B
    Date: 2003
    Issue Date: 2009-11-30 11:32:55 (UTC+8)
    Abstract: The work of fabricating a piezoelectric (PZ) immunosensor for the detection of staphylococcal enterotoxin B (SEB) is presented in this paper. Three different immobilization methods using anti-SEB antibody onto a gold electrode of the PZ have been conducted. The electrode coated with polyethyleneimine (PEI) has shown the best result. The fabricated PZ sensor can be used for SEB determination in the range of 2.5-60 mug/ml with a correlation coefficient of 0.997. Milk samples spiked with various concentrations of SEB gave an average of 111% recovery of the toxin. The SEB assay is specific. For example the presence of staphylococcal enterotoxin A (SEA) at 40 mug/ml gave 6.44% of the signal while staphylococcal enterotoxin D (SED) appeared to give no detectable signal. After regeneration with 1.2 M NaOH, the coated crystal could be reused three times with retention of 66% of the initial signal. The crystal has also been found to be stable for 3 days when stored at 4 degreesC in a dry atmosphere without appreciable loss of activity. (C) 2003 Elsevier Science B.V. All rights reserved.
    Relation: BIOSENSORS & BIOELECTRONICS Volume: 18 Issue: 12 Pages: 1479-1483
    Appears in Collections:[Graduate Institute of Biotechnology ] journal articles

    Files in This Item:

    File Description SizeFormat
    index.html0KbText590View/Open


    All items in CCUR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback