文化大學機構典藏 CCUR:Item 987654321/2874
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 46962/50828 (92%)
造訪人次 : 12453329      線上人數 : 655
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於CCUR ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/2874


    題名: In vitro reduction of trypsin inhibitor by purified NADPH/thioredoxin system from sprouts of sweet potato (Ipomoea batatas (L) Lam.) storage roots
    作者: Huang DJ
    Chen HJ
    Hou WC
    Chen TE
    Lin YH
    貢獻者: 園生系
    關鍵詞: trypsin inhibitor
    NADPH/thioredoxin system
    sweet potato
    NADPH/thioredoxin reductase
    日期: 2004
    上傳時間: 2009-11-27 11:51:06 (UTC+8)
    摘要: Sweet potato (Ipomoea batatas (L.) Lam) storage roots were sprouted in the dark for two months, and the sprouts were used as source materials for purification of thioredoxin reductase successively via the DE-52, Sephadex G-75, and 2',5'-ADP Sepharose 4B column chromatography. Thioredoxin reductases with a molecular mass of ca 33 kDa were obtained. The purification was 813-fold and the yield was 2.5%. The effect of thioredoxin reductase on trypsin inhibitor activity (TIA) was analyzed with a TIA assay method. Native, oxidized form of trypsin inhibitors (TIs) inhibited trypsin activity to 24% of its original value. Trypsin activity recovered to 38 and 46% of its original value, respectively, when TI was reduced in advance by NTR system and DTT. The data indicate that NTR-reduced TIs lost the capacity to inhibit trypsin activity in vitro. mBBr/SDS-PAGE was used to analyze the reduction of TIs on gels. The reduced TIs were more sensitive to heat denaturation, and exhibited increased digestibility by partially purified proteases from sweet potato sprouts. Based on these results, we conclude that the NADPH/thioredoxin system is likely associated with the reduction of TIs and initiation of degradation and re-mobilization of stored TIs during sprouting of sweet potato storage roots. Meanwhile we may expect sprouts to be a better protein source for consumption than SP storage root itself. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
    關聯: PLANT SCIENCE Volume: 166 Issue: 2 Pages: 435-441
    顯示於類別:[園藝暨生物技術學系] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbText628檢視/開啟


    在CCUR中所有的資料項目都受到原著作權保護.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋