The purpose of this study was to investigate the soil organic matter content from stands of Hua-Lin Ecological Garden by loss on ignition method. This study sampled soils from Chamaecyparis formosensis stand, Araucaria cunninghamii D. Don stand, pine stand, bamboo stand, grassland, hardwood stand of ecological management area, grassland, and Acacia confusa secondary forest of Hua-Lin Ecological Garden. The soils sampled were divided into two depths: 0-15 cm and 15-30 cm. Soil moisture content and soil organic matter content were investigated. The results showed highly significant differences (P<0.0001) between forest stands for both soil moisture content and soil organic matter content from soils sampled in June, 2011. Soil moisture content from soils sampled in January of 2012 both showed highly significant differences in forest stands and in soil depths; however, soil organic matter content showed significant and highly significant difference in forest stands and in soil depths, respectively. Soil from hardwood stand had highest soil moisture content and soil organic matter content, and grassland the lowest. Compared to the lower soil depth (15-30cm), the upper soil depth (0-15cm) had higher soil moisture content and soil organic matter content.