海芋健康種球量產技術之開發(II)

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Title:	海芋健康種球量產技術之開發(II) Development on Healthy Rhizome Bulb Production of Zantedeschia aethiopica L.
Authors:	陳麗如熊同銓黃子彬
Contributors:	園藝暨生物技術學系
Keywords:	種苗白花海芋茉莉酸根莖種球形成 Plantlet Zantedeschia aethiopica Jasmonic acid Rhizome Bulb formation
Date:	2014-01
Issue Date:	2014-10-23 13:19:54 (UTC+8)
Abstract:	白花海芋 (Zantedeschia aethiopica L.) 是陽明山極具重要性的經濟花卉作物，為促進海芋產業的永續發展，本二年期研究計畫建立海芋的健康種球量產技術。第一年已建立白花海芋芽體形成及發育二階段的芽體量化流程，以根莖芽基盤為培植體，在含0.5 mg L-1 TDZ及0.5 mg L-1 BAP的MS基本培養基，經光照培養4周後，平均可誘導5個以上的芽體形成，接續分切芽體繼代培養於MS基本培養基，光照培養4周，芽體發育後再重覆分切芽基盤進行芽體增殖。茉莉酸經由刺激細胞分裂及促進細胞擴增參與控制球根植物的球根形成。第二年將接續探討不同濃度的蔗糖與茉莉酸組合對白花海芋芽體形成種球的影響，以找出種球形成的關鍵因素。未來將可應用於開發白花海芋量產健康根莖種球的技術平台，並可供應農民健康種球更新的來源。 White calla lily ( Zantedeschia aethiopica L.) is an important economic flower crop in Yang-Ming-Shan National Park. For sustainable development of calla lily industry, we plan to develop the production of healthy rhizome bulb in this two-year plan. In the first year we have established a two-stage process of shoot proliferation in white calla lily including shoot formation and development. Shoot disc was used to induce formation of more than 5 shoots on MS basal medium containing 0.5 mg L-1 TDZ and 0.5 mg L-1 BAP after 4 weeks of culture in the light. Then shoots were separated individually and cultured on MS basal medium for shoot development in the light. After 4 weeks of shoot development, shoot disc can be cut for next two-stage shoot proliferation. Jasmonic acid was reported to control bulb and tuber formation mediated by cell division and expansion. In the second year we continue to develop mass production system of healthy bulblet formation of white calla lily. The combination of different concentrations of sucrose and jasmonic acid will be used to find out a suitable condition for bulblet development. The mass propagation of healthy rhizome bulb of white calla lily could serve as plant material source for growers to renew bulbs in the future.
Appears in Collections:	[Department of Horticulture] project

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