| 摘要: | Antrodia camphorata aka A. camphorata is a basidiomycete in Polyporaceae, which causes brown heart rot in Cinnamomun kanehirai hay (Lauraceae), has been utilized in traditional Taiwan medicine. Crude extracts of A. camphorata in different models, in vivo and in vitro studies, show its pharmacological effects, such as anti-cancer activities, anti-inflammatory effects, anti-hepatitis B virus replication, anti-oxidant activities, hepatoprotective activities, prevention of liver fibrosis, neuroprotective effects, antihypertensive effects and vasorelaxation effects. It was tried to make a solid medium of food-ingredient, instead of Cinnamomun kanehirai wood chuck, to raise the A. camphorata fruiting body to be a better substituted source to produce the functional food, A. camphorata. In present study, A. camphorata raised on different types of food-ingredient media was used to figure out the best culture condition with high nutraceutical products such as triterpenoids and polysaccharide concentration, and used to compare with the submerged culture of A. camphorata for their nutritional contents. Nutraceutical effects were evaluated by measuring their antioxidant effects (DPPH assay) after extracting by methanol or hot water from A. camphorata. Results showed oat-meal cultured A. camphorata had the thickest fruiting body and highest dried weight (average= 1.3g/ culture plate). Component analysis showed oat-meal cultured A. camphorata contained 9% triterpenoid. Food-ingredient solid medium had high concentration of triterpenoid produced by A. camphorata fruiting body (9%), which was higher than that produced by submerged-cultured mycelia (1.73%). This could be a high efficiency and cheaper way to produce A. camphorata. The extracts of A. camphorata polysaccharides and triterpenoids was also investigated for their effects on stimulating cytokine expression in mouse splenocytes, of which ten cytokines, IL-1, IL-2, IL-4, IL-6, IL-12, IFN-, TNF-α, GMCSF, G-CSF, and M-CSF, were chosen and analyzed. Results showed that most of them were significantly expressed by treating with the extracts of A. camphorata. |
