Active recombinant thioredoxin h protein with antioxidant activities from sweet potato (Ipomoea, batatas [L.] lam tainong 57) storage roots

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Title:	Active recombinant thioredoxin h protein with antioxidant activities from sweet potato (Ipomoea, batatas [L.] lam tainong 57) storage roots
Authors:	Huang DJ Chen HJ Hou WC Lin CD Lin YH
Contributors:	園生系
Keywords:	sweet potato thioredoxin h cDNA sequence gene expression recombinant protein antioxidant
Date:	2004
Issue Date:	2009-11-23 11:27:29 (UTC+8)
Abstract:	Recombinant thioredoxin h (Trx2) overproduced in Escherichia coli(M15) was purified by Ni2+-chelated affinity chromatography. The molecular mass of Trx2 is similar to1.4 kDa as determined by sodium dodecyl sulfate- polyacrylamide gel electrophoresis. Total antioxidant status, 1,1-diphenyl-2-picrylhydrazyl (DPPH) staining, reducing power method, Fe2+-chelating ability, ferric thiocyanate (FTC) method, and protection of calf thymus DNA against hydroxyl radical-induced damage were studied. The thioredoxin h protein with a concentration of 12.5 mg/mL exhibited the highest activity (expressed as 0.37 +/- 0.012 mM ABTS(.) radical cation being cleared) in a total antioxidant status test. In the DPPH staining thioredoxin h appeared as white spots when it was diluted to 50 mg/mL (a final amount of 15 mug). Like the total antioxidant status, the reducing power, Fe 2(+) -chelating ability, FTC activity, and protection against hydroxyl radical-induced calf thymus DNA damage were found with the thioredoxin h protein. It was suggested that thioredoxin h might contribute to its antioxidant activities against hydroxyl and peroxyl radicals.
Relation:	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Volume: 52 Issue: 15 Pages: 4720-4724
Appears in Collections:	[Department of Horticulture] journal articles

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