Bimolecular triplex formation between 5 '-d-(AG)(n)T-4(CT)(n) and 5 '-d-(TC)(n) as functions of helix length and buffer

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题名:	Bimolecular triplex formation between 5 '-d-(AG)(n)T-4(CT)(n) and 5 '-d-(TC)(n) as functions of helix length and buffer
作者:	Chin TM;Chang CM;Huang HW;Lo LL
贡献者:	化學系
日期:	2004
上传时间:	2009-11-23 11:25:35 (UTC+8)
摘要:	It was observed that a group of unusually stable DNA hairpins (Hn: 5'-d-(AG)(n)T-4(CT)(n), n = 2-4) were directed to homopyrimidine sequences (Pn: 5'-d-(TC)(n)) by py . pu . py-type triplex formation, resulting in high binding affinity and specificity. The spectroscopic results (UV and CD) showed that the short bimolecular triplex Hn:Pn could be formed in acidic conditions (pH 4.5-6.0) as helix length n > 2, and further extending to neutral pH as n = 4. This hairpin strategy for recognition of a pyrimidine strand has a substantial binding advantage over either the conventional linear analog or simple Watson-Crick complement. Triplex stability of Hn with Pn is not only pH-dependent, as expected for triplexes involving C+ GC triads, but also sensitive to the buffer. The triplex H4:P4 was formed in the phosphate buffers of pH 6.0-7.0 but already dissociated above pH 6.5 in the buffer of cacodylate, MOPSO or PIPES. By contrast, the nature of a buffer had no major influence on stability of a hairpin duplex. Here we provide a simple triplex system, and the data presented here may be useful in defining the experimental conditions necessary to stabilize triplex DNA.
關聯:	JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS Volume: 22 Issue: 1 Pages: 35-43
显示于类别:	[化學系所] 期刊論文

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