兒茶素補充對敗血症大白鼠多重器官損傷之效應

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Title:	兒茶素補充對敗血症大白鼠多重器官損傷之效應 Effects of Catechin Supplementation on Multiple organs Injury in Septic Rats
Authors:	王鼎耀
Contributors:	生活應用科學研究所
Keywords:	sepsis multiple organ injury(MOI) cecal ligation and puncture(CLP) catechin
Date:	2006
Issue Date:	2014-06-24 11:11:00 (UTC+8)
Abstract:	Sepsis is a severe clinical problem with high mortality rate. The septic responses including systemic hypotension, disseminated intravascular coagulation(DIC),abnormal coagulation and multiple organ injury(MOI) ensued from the septic shock response are the main reasons that the patient causes death. Recent studies had demonstrated that reactive oxygen species(ROS) play an important role in rate development of multiple organs injury during sepsis. Excess ROS production cause lipid peroxidation of cell membrane, and further to damage cell membrane structures and functions. Several studies had also confirmed that some kinds of antioxidants protest rats against sepsis-induced multiple organ injury, for example, sesame oil and its active componeuts(sesamol). There fore, management of oxidative stress could be an important issues in care of sepsis. There has been abundant research showing that replenishing green tea catechin has many benefits. It not only reduces plasma total cholesterol and low density lipoproteins(LDL) concentration, it can also moderate the incidence of cardiovascular disease and retard damage to animal antioxidants. Therefore, not only does EGCG of catechin polyphenol have strong anti-inflammatory effects, it also inhibits cancer cell growth or accelerates cancer cell apoptosis. Catechin is one of isoflavone with a high ROS-scavenging effect. The aims of present study were to evaluate the effects of catechin supplementation on lipid peroxidation and MOI of septic rats. In this project we used male SD rats subjected to cecal ligation and puncture operations to mimic peritoneal sepsis. Male SD rats(300g~350g) were supplemented with saline(1mL/kg BW) or catechin(40mg/kg BW) for 4 days. Following sepsis induction, survival rate, blood oxygenation indicator(lactate) and biochemical marker for liver or renal functions were measured like blood urea nitrogen(BUN), creatinine(CRE), alanine transaminase(ALT), total bilirubin(TBIL). Next, we also examined oxidative damage indicator, lipid peroxidation products, malondialdehyde(MDA) in plasma, liver and kidney. Finally, we analyzed endogenous oxidant/antioxidant enzymes system, including activities of superoxide dismutase(SOD), catalase(CAT) and glutathione peroxidase(GPx) in plasma, liver and kidney specimens. Our data showed that at 18h-post CLP, the survival rate of catechin-supplemented septic rats(100% v.s. 75%) Plasma lactate levels, lipid peroxidation marker(MDA content) in plasma, liver and kidney samples were all lower in septic rats receiving catechin supplementation attenuated heptic and renal injury in septic rats. Finally, CLP operations reduced SOD,CAT and GPx activities of liver and kidney samples at 18h post operatio; but catechin- supplemented septic rats exhibited higher antioxidant activity than saline-supplemented rats. These results suggesting that CLP induced oxidative stress more strongly in saline-supplemented rats, which may account for the more severe hepatic and renal damage by lipid peroxidation. Enhanced SOD, CAT and GPx activities of liver and kidney were found in rats receiving catechin supplementation. In conclusion, catechin supplementation attenuated lipid peroxidation and improved organs function during CLP-induced sepsis.
Appears in Collections:	[Department of Applied Science of Living & Graduate Institute of Applied Science of Living ] thesis

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