Sweet potato (Ipomoea batatas (L.) Lam. 'Tainong 57') storage root mucilage exhibited angiotensin converting enzyme inhibitory activity in vitro

文化大學機構典藏 CCUR > 環境規劃暨生物資源學院 > 農學院 > 園藝暨生物技術學系 > 期刊論文 > Item 987654321/2736

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题名:	Sweet potato (Ipomoea batatas (L.) Lam. 'Tainong 57') storage root mucilage exhibited angiotensin converting enzyme inhibitory activity in vitro
作者:	Huang, Dong-Jiann Hou, Wen-Chi Chen, Hsien-Jung Lin, Yaw-Huei
贡献者:	園生系
关键词:	angiotensin converting enzyme (ACE) mucilage sweet potato
日期:	2006
上传时间:	2009-11-16 12:01:36 (UTC+8)
摘要:	Sweet potato (Ipomoea batatas (L.) Lam. 'Tainong 57') storage root mucilage was extracted (crude mucilage) and further purified by SDS and heating treatment (purified mucilage). Purified mucilage treated with 2-mercaptoethanol moved as a single band in SDS-PAGE. This purified mucilage was active to inhibit angiotensin converting enzyme (ACE) as shown by spectrophotometric method in a dose-dependent manner (28.7 to 59.8% ACE inhibition, respectively, by 50 to 400 mu g/mL mucilage) using (N-(3-[2-furyl] acryloyl)-Phe-Gly-Gly) (FAPGG) as a substrate. The concentration of mucilage required for 50% inhibition (IC50) of ACE activity was 364.5 mu g/mL while that of captopril was 10 nM (8.68 mu g/mL). The commercial polysaccharide pectin (50 to 400 mu g/mL) showed no inhibitory activity against ACE. When using fluorescent silica TLC to detect FAPGG and FAP, the results also showed that mucilage inhibited ACE. The mucilage showed mixed type inhibition against ACE, and the Michaelis constant in the presence of mucilage was 12 mM. We suggest that consumption of sweet potato storage root mucilage may benefit people's health.
關聯:	BOTANICAL STUDIES Volume: 47 Issue: 4 Pages: 397-402
显示于类别:	[園藝暨生物技術學系] 期刊論文

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